The paper aims to identify the determinants that influence entrepreneurial intention among National Economics University graduates. For the sample size, we conducted a questionnaire survey of 250 full-time third-year undergraduates at the National Economics University, with convenience sampling technique. Of those 250 undergraduates, 150 were Business Administration students and 100 Economy Management students. After eliminating invalid responses due to lack of information or low quality information, 215 responses were used (93.07% of respondents) for data analysis. The results show that the factor "Perceived desirability" has the strongest impact on the intention to start a business. It is followed by the factor "Perceived feasibility" in which the "ability to search and plan for start-up" has a stronger influence on undergraduates' entrepreneurial intention than the "leadership and ability to overcome adversity". The hypothesis of a positive relationship between undergraduates' propensity to act and their intention to start a business is also supported in this study. Based on the results, we suggest some recommendations for the university and State management agencies to nurture and develop entrepreneurial intention of undergraduates, forming the young generation of potential entrepreneurs to contribute to the development of emerging countries such as Vietnam, the case study.
Positive control (or standard) is an indispensable ingredient in molecular biology assays widely used for the quantification of nucleic acid. The commonly used standards are plasmid DNA, cDNA, or naked RNA, which are unstable and easily degraded by nucleases in the surrounding environment; this might affect the accuracy of quantitative results. In this study, the authors designed and created a positive control for the hepatitis C virus (HCV) quantification based on armored RNA technology. The 5’UTR non-encoding sequence of HCV was cloned into the BH20 plasmid. Armored RNA HCV (AR-HCV) was induced for expression in the E. coli BL21 (DE3) by the addition of an IPTG inducer. AR-HCV was collected by sucrose density gradient ultracentrifugation followed by gel filtration chromatography using Superdex 75 column. Created AR-HCV was determined the concentration and examined the formation of pseudo viral particles by transmission electron microscopy (TEM). Stability assessment of AR-HCV to DNase and RNase treatment simultaneously has demonstrated its ability to resist these nucleases. Moreover, AR-HCV is stable over time and storage conditions. Strikingly, AR-HCV can be directly added to the specimen, allowing better and more accurate control of the whole quantitative procedure of HCV.
Backgroud: Gac is a fruit containing many antioxidants, especially lycopene, with high concentration. However the variety and value of products from gac are limited. We introduce a modified method to produce lycopene and others carotenoids from gac oil towards available and safe applications in nutraceutical and cosmetics. Materials and method: We study the extraction with different parameters and limit at the saponification of gac oil using less toxic substances such as ethanol, propylene glycol, postasium hydroxide and sodium chlorid. Results: Concentrations of lycopene and β-carotene determined silmutaneously by UVVis spectrophotometer present that the efficiency reaches 65.07% as 20 g of gac oil is saponified with 8 g KOH 12 mol/L in 120 mins. The purity of product is 89.02%. Conclusions: This process could minimize toxic residue in the powder after the precipitation, filtration and washing. Besides, the method is stable and applicable to the mass production. Key words: Carotenoids, Lycopene, safe extraction, UV Vis silmutaneous concentration determination
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