This systematic review aimed to verify the empirical evidence of the application of the eye tracking technique in depressive disorder. The PubMed, PsyInfo and Web of Science databases were used, using the following descriptors: ((Depression OR "Affective Disorders") AND ("Eye Tracking" OR Eye-Tracking OR "Eye Movements" OR "Eye Tracker" OR "Eye Gaze")). According to the eligibility criteria, sixteen relevant studies were identified and its methods, results and their main limitations were verified. The results suggest an increase in latency and reaction time to detect complex stimuli, e.g. the recognition of emotional facial expressions. In addition, greater numbers of fixations in stimuli of negative emotional content were observed. The findings demonstrate that changes in eye movement measurements in depressive disorder are associated with an attentional bias mechanism. It is concluded that Eye Tracking is a useful tool for psychophysiological assessment of depressive disorder.