True tendon regeneration in human patients remains a vision of musculoskeletal therapies. In comparison to other mesenchymal lineages the biology of tenogenic differentiation is barely understood. Specifically, easy and efficient protocols are lacking that might enable tendon cell and tissue differentiation based on adult (stem) cell sources. In the murine mesenchymal progenitor cell line C3H10T½, overexpression of the growth factor bone morphogenetic protein 2 (BMP2) and a constitutively active transcription factor, Smad8 L + MH2, mediates tendon cell differentiation in vitro and the formation of tendon-like tissue in vivo. We hypothesized that during this differentiation secreted factors involved in extracellular matrix formation exert a major impact on tendon development. Gene expression analyses revealed four genes encoding secreted factors that are notably upregulated: periostin, C-type lectin domain family 3 (member b), RNase A4, and follistatin-like 1. These factors have not previously been implicated in tendon biology. Among these, periostin showed a specific expression in tenocytes of adult mouse Achilles tendon and in chondrocytes within the nonmineralized fibrocartilage zone of the enthesis with the calcaneus. Overexpression of periostin alone or in combination with constitutively active BMP receptor type in human mesenchymal stem cells and subsequent implantation into ectopic sites in mice demonstrated a reproducible moderate tenogenic capacity that has not been described before. Therefore, periostin may belong to the factors contributing to the development of tenogenic tissue.

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Object Most upper cervical spine injuries are able to heal conservatively by halo vest application. The acceptance of the halo is different among patients due to the weight of the apparatus and the limited mobility it causes. Additionally, the fracture healing rate in older patients seems to be inferior to that in younger patients, which would make an operative stabilization procedure more appropriate for the elderly. Furthermore, the risk of complications is assumed to be higher in older people. The purpose of this retrospective study was to find out if there are differences in the clinical and radiological results and in complication rates between 2 patient groups with upper cervical spine injuries and halo vest treatment with special interest of their age group. Methods A total of 29 patients with upper cervical spine injuries were treated by halo vest application. The clinical and radiological results and the complication and revision surgery rates were identified. Patients were divided into 2 groups (18 patients were < 65 years and 11 were > 65 years), and the results were evaluated to examine whether there were any differences. Results The clinical and radiological results and the complication rate were not statistically significantly different between the 2 patient groups; however, there was a tendency for a longer time interval for fracture healing and more complications in the elderly people. Conclusions If the conditions for conservative treatment of upper cervical spine injuries with halo fixation are right, the clinical and radiological results are good and almost similar in patients regardless of their age, although there is a tendency for more complications in older people.

The analysis of arthroscopic biopsies represents a helpful tool to verify or rule out a PJI in selected patients. Nevertheless, minimally invasive diagnostic tools (e.g., laboratory analysis and aspiration) should be utilized beforehand.

In this study, the success of cup positioning in total hip arthroplasty (THA) using an imageless navigation system was analyzed (1) during the learning period and (2) after the learning period for using the navigation system. Sixty THAs were performed in which threaded cups were placed with use of a computer-assisted navigation device (B. Braun Aesculap, Tuttlingen, Germany). Half of the procedures (30), group A, were done by the same surgeons under the learning curve for using the navigation system; the other half (30), group B, were done by surgeons who were no longer considered under the learning curve. Intraoperative acetabular component parameters (inclination, anteversion) for both groups were compared with postoperative radiographic alignment values. In group A, significant differences were seen between intraoperative and postoperative cup orientation. In group B, no significant differences were seen between intraoperative and postoperative cup orientation. Additionally, the percentage of outliers decreased in group B. Operating and anesthesia times significantly decreased with the surgeon's experience in imageless cup navigation. There was an individual increase of precision during the learning curve for all surgeons. Imageless navigation is a dependable and accurate method of cup positioning during THA. However, accuracy of cup placement and length of operating room time were affected by surgeons' experience using the system. Imageless navigation may lead to a reduction in the length of the learning curve for surgeons beginning to perform THAs, improvement in the surgeon's ability to perform this procedure safely, and minimization of outliers.