Here, 4 polymerase chain reaction (PCR) assays are compared to test for the presence of avian malaria, including both the Plasmodium and Haemoproteus genera, in 29 different species of African rainforest birds. Two of these PCR assays use primer sets that amplify fragments of the cytochrome b (cyt b) gene of Plasmodium; the other 2 target the 18S ribosomal subunit gene. These PCR assays were performed using genomic DNA extracted from blood and subsequently compared with the results obtained by microscopic examination of blood smears taken from the same individuals. The 2 primer sets amplifying the cyt b gene were found to perform more reliably than those that target the 18S rRNA gene and yielded a substantial number of positive samples that were undetected by blood smear analysis. Of all the individuals screened by PCR, 40% tested positive for avian malaria, whereas 27% tested positive by blood smear analysis. Although sequence variation in the parasites may prohibit the specific alignment of primers and the subsequent PCR amplification of some individuals, PCR, once optimized, is faster, cheaper, and more reliable than blood smear analysis for large-scale screening.

The topological structure associated with the branch point singularity around an exceptional point (EP) can provide tools for controlling the propagation of light. Through use of graphene-based devices, we demonstrate the emergence of EPs in an electrically controlled interaction between light and a collection of organic molecules in the terahertz regime at room temperature. We show that the intensity and phase of terahertz pulses can be controlled by a gate voltage, which drives the device across the EP. Our electrically tunable system allows reconstruction of the Riemann surface associated with the complex energy landscape and provides topological control of light by tuning the loss imbalance and frequency detuning of interacting modes. Our approach provides a platform for developing topological optoelectronics and studying the manifestations of EP physics in light–matter interactions.

We present a simple theory of thermoelectric transport in bilayer graphene and report our results for the electrical resistivity, the thermal resistivity, the Seebeck coefficient, and the Wiedemann-Franz ratio as functions of doping density and temperature. In the absence of disorder, the thermal resistivity tends to zero as the charge neutrality point is approached; the electric resistivity jumps from zero to an intrinsic finite value, and the Seebeck coefficient diverges in the same limit. Even though these results are similar to those obtained for single-layer graphene, their derivation is considerably more delicate. The singularities are removed by the inclusion of a small amount of disorder, which leads to the appearance of a "window" of doping densities 0 < n < nc (with nc tending to zero in the zero-disorder limit) in which the Wiedemann-Franz law is severely violated.

Here we present a simple extension to the age-old Kronig-Penney model, which is made to be bipartite by varying either the scatterer separations or the potential heights. In doing so, chiral (sublattice) symmetry can be introduced. When such a symmetry is present, topologically protected edge states are seen to exist. The solution proceeds through the conventional scattering formalism used to study the Kronig-Penney model, which does not require further tight-binding approximations or mapping into a Su-Schrieffer-Heeger model. The topological invariant for this specific system is found to be the winding of the reflection coefficient, ultimately linked to the system wavefunction.The solution of such a simple and illustrative 1D problem, whose topological content is extracted without requiring further tight-binding approximations, represents the novel aspect of our paper. The cases in which chiral symmetry is absent are then seen to not host topologically protected edge states, as verified by the behaviour of the reflection coefficient and the absence of winding.

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