The nature of nutrient limitation in large areas of temperate forest may be changing due to human activities. As N availability in these forests increases, other nutrients could increasingly constrain productivity and other ecosystem processes. To determine the nature of nutrient limitation (N, P, and Ca) in forest soils exhibiting differing N availability, we conducted three field studies in the Fernow Experimental Forest, West Virginia, USA. The first used a ubiquitous herbaceous species, Viola rotundifolia, to compare indices of N availability to the activity of root-associated phosphomonoesterase (PME) activity at two spatial scales. The second study used fertilized, root in-growth cores to assess the extent of N, P, and Ca limitation. Finally, we measured the root-associated PME activity of V. rotundifolia growing in experimental plots that have received various combinations of nutrient additions and harvest treatments. For entire watersheds, stream water nitrate concentrations were positively related to PME activities (R2 = 0.986). For small plots, PME activities were positively associated with soil nitrate availability (R2 = 0.425), and to a lesser extent with the leaf N concentrations (R2 = 0.291). Root growth into microsites fertilized with P was greater than growth into microsites fertilized with either N or Ca, especially in watersheds with high N availability. Experimental additions of N increased the root-associated PME activity of V. rotundifolia, supporting the causality of the relationship between N availability and PME activity. Collectively, our results indicate that, as N availability increases, P becomes increasingly limiting at the sites examined. Understanding how nutrient limitations change during N saturation should improve ecosystem models and better inform our attempts to mitigate any undesired effects.

Abstract. Rhizosphere microbial populations may increase bioremediation of soil contaminated with organic chemicals. A growth chamber study was conducted to evaluate rhizosphere microbial populations in contaminated and non-contaminated soil. Alfalfa (Medicago sativa L.) and alpine bluegrass (Poa alpina L.) were grown in soil containing a mixture of organic chemicals for 14 weeks. The equal millimolar mixture of hexadecane, (2,2-dimethylpropyl)benzene, cis-decahydronaphthalene (decalin), benzoic acid, phenanthrene, and pyrene was added at levels of 0 and 2000 mg/kg. Organic chemical degrader (OCD) populations were assessed by a Most-Probable-Number technique, and bacteria and fungi were enumerated by plate count methods. Different methods for expressing OCD rhizosphere populations were investigated to determine the effect it had on interpretation of the results. At 9 weeks, the OCD numbers were significantly higher in rhizosphere and contaminated soils than in bulk and non-contaminated soils, respectively. Alfalfa rhizosphere OCD levels were 4 x 107/g for contaminated and 6 x 106/g for non-contaminated soils. Bluegrass rhizosphere OCD levels were 1 • 107/g and 1 x 106/g in contaminated and non-contaminated soils, respectively. Selective enrichment of OCD populations was observed in contaminated rhizosphere soil. Higher numbers of OCD in contaminated rhizospheres suggest potential stimulation of bioremediation around plant roots.

Total joint replacements traditionally employ ultra high molecular weight polyethylene (UHMWPE) as a bearing material due to its desirable material properties and biocompatibility. Failure of these polyethylene bearings can lead to expensive and risky revision surgery, necessitating a better understanding of UHMWPE’s tribological properties. A six-station rolling/sliding machine was developed to study the behavior of accelerated-aged UHMWPE in cylinder-on-cylinder contact. The normal load and sliding/rolling ratio in the oscillatory contacts can be controlled separately for each test station, as can the liquid test environment. Fatigue tests were run on the machine with UHMWPE versus cobalt-chrome cylinders in a distilled water environment at normal contact pressures of approximately 20 MPa. All specimens failed by subsurface cracking during tribotesting on the machine, and the failures were similar to those that occur in-vivo. The fatigue behavior of the polymer was analyzed to determine its relationship to oxidation and stress state in the rolling/sliding cylinder. At the 20 MPa test load, the number of cycles to fatigue failure by subsurface cracking was inversely proportional to the oxidation level. Analysis of the stress levels through the bulk of the polyethylene specimens and their relationship to the material properties provide insight as to why cracks initiate and propagate subsurface.

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