Objectives: Sudan IV dye (S4D), has been reported to enhance the color of food products example palm oil, despite the health issues associated with these colorants. The potent antioxidant attribute of Curcuma longa facilitates its capacity to protect the kidney against deleterious attacks by free radicals. This study investigated the rejuvenating potentials of the extract of C. longa by S4D induced hepato-renal injury in rats. Material and Methods: Twenty-five female albino rats were distributed into five groups, as follows: Group 1 received distilled water and diet only, Group 2 received 10% S4D+100 mg C.L, Group 3 received 15% S4D +100 mg C.L, while Groups 4 and 5 received 200 mg and 250 mg C.L, respectively. After 3 weeks of administration of Curcuma treatment, animals were sacrificed; liver, kidney, and small intestine were collected for analyses. Aspartate and alanine aminotransferases, gamma-glutamyl transferase, glutathione-s-transferase, alkaline phosphatase, and lactate dehydrogenase, as well as some selected electrolytes were measured. Results: Results show that treatment with 200 mg and 250 mg/kg significantly (P < 0.05) rejuvenated these enzyme biomarkers while modulating the physiological levels of the serum electrolytes and kidney markers. Conclusion: C. longa exhibited a significant antioxidant potential that sustain the cells from the effect of S4D toxicity. Hence, the results indicate a possible ameliorative mechanism of the plant extract against S4D- induced hepato-renal injury. Therefore, the inclusion of C. longa into our daily diet might limit the effect of the hepato-renal injury.