Metal nanoparticles are widely utilized in biotechnology and biomedicine for various applications, including medication delivery, imaging, and bacterial growth control. Silver nanoparticles (AgNPs) were synthesized by bacteria, fungi, algae, and plants. The Study aimed to synthesize nanomaterial with a cost-effective, environmentally friendly, and the uses of AgNPs as antibacterial (against pathogenic bacteria) and anticancer (on MCF7 cell line). In this Study, bacteria were collected from different soil samples. Isolated, purified by selective media, identification genotypically by 16rRNA sequencing analysis, then compared with NCBI, GenBank as Microbacterium sp. Biosynthesis of silver nanoparticles using Microbacterium for extracellular synthesis by reducing silver ions to silver nanoparticles. The color change to brown and reddish-brown was the first indication of the AgNPs formation; physical characterization using UV-Visible spectroscopy showed a wavelength in 489 nm, while X-ray diffraction (XRD) revealed that the silver nanoparticles were crystalline; transmission electron microscope (TEM) image showed that AgNPs spherical in shape with an average diameter of around 50 nm, in SEM (Scanning electron microscope) AgNPs formed with a diameter of 41-44 nm, spherical and uniform, while Energy-dispersive X-ray show very high silver peaks. Bioactivity of AgNPs by antimicrobial on pathogenic bacteria, which collected from Al- Sadr hospital in Misan (identified by using VITEK). This experiment showed that the inhibition zone was rung from (6- 38mm) on pathogenic bacteria; it was tremendous compared with antibiotics (Gentamycin in this project ranged from(7-28.5mm). Antitumor activity of extracellular biosynthesized AgNPs was determined using the MTT test against breast cancer cells (MCF7 cell line), which showed very high results. AgNPs inhibition breast cancer cell line by about 81% at 100ug/ml, indicating that the rate is outstanding. Finally, different biomedical approaches can benefit from AgNPs as antibacterial agents and anticancer agents with many results. Keywords. Silver Nanoparticles, Biosynthesis, Antibacterial, and Antitumor.