Studies related to ants found in hospital environments have aroused interest in their role as mechanical vectors of pathogenic microorganisms. The objective of the current research was to determine the species composition and bacterial contamination of ant species found in a public hospital in the eastern Amazonian region. Ants were captured using bait containing honey and sterilized sardines in 15 locations within the Macapá Emergency Hospital, Amapá. Ants were identified morphologically using specific keys. Bacteria were first inoculed in a Brain Heart Infusion broth and then plated on 5% Agar with blood or MacConkey media. Bacterial species were identified through biochemical procedures. In total, 9,687 ants were collected, with 69.8% from the dry season and 30.2% from the rainy season. Nine species of ants were identified belonging to three subfamilies: the Monomorium pharaonis (Linnaeus 1758) being the most common, comprising 39.2% of the total specimens. Only one ant species was found in each bait, facilitating microbiological analyses. In total, 92 bacteria isolates were identified comprising 12 species. Pseudomonas aeruginosa Schroeter 1872 (Pseudomonadales: Pseudomonadaceae) was pathogenic bacteria, most frequently isolated, comprising 10.9% of the positive samples. The most contaminated ant in the study was M. pharaonis with 38.3%. It was the dominant ant species in this hospital environment. Its wide prevalence, forage day and night of this vector in hospital facilitated bacterial contamination. The presence of bacteria on ants may be associated with the dissemination of pathogens which cause hospital infections, making pest control a necessity in these institutions.

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Siparuna guianensis (Laurales: Siparunaceae) has a terpene-rich essential oil with great potential for larvicides. The poor water miscibility of their compounds makes nano-emulsions of great interest for novel bioactive systems, including for control of Aedes aegypti (Diptera: Culicidae). This species is adapted to urban environments with important role in the epidemiology of some arboviruses such as dengue, chikungunya fever, zika, and urban yellow fever. The aim of the present study was to evaluate the feasibility of nano-emulsification to affect Ae. aegypti larvae. An optimal system was achieved by using a nonionic single surfactant, highlighted by its satisfactory size distribution profile. Moreover, improved larvicidal activity in comparison to bulk essential oil can be observed for the nano-emulsions. The estimated LC50 and LC90 values after 24 h of treatment of larvae with the essential oil were, respectively, 86.5232 and 134.814 µg/ml, while the estimated LC50 and LC90 value after treatment with the nano-emulsion were 24.7572 and 75.2452 µg/ml, respectively. The utilization of a simple technique to produce a fine nano-emulsion opens perspective for further integrative practices of mosquito control and giving value to this Amazon plant species may encourage its sustainable use and contribute to conservation policies.

The Odonata Order comprises one of the largest groups of aquatic insects, 7,000 species are described worldwide, with approximately 860 species registered in Brazil, around 14% of the global fauna known to date. However, there are still great gaps in the knowledge of Odonata fauna in many areas of Brazil. This study aims to present the results of the survey of Odonata species sampled in three counties in the state of Amapá. The state is located in the north of the country, inserted in the Amazon Biome. Odonata were caught between January and December 2018, with 472 specimens being sampled, belonging to seven families, 36 genera and 53 species. In total, 27 of the species found during the study correspond to new records for the state of Amapá. Due to the lack of information on the diversity of the Odonata order in Amapá, the list of species presented should serve as input for new studies contributing to the knowledge of the order in the state.

El Amapá está ubicado en el norte de Brasil, numerosos grupos están poco estudiados en el estado, entre ellos el orden Odonata. El objetivo del presente trabajo es presentar datos sobre la diversidad de los Zygopteros de Amapá. El muestreo se realizó entre los años 2018 y 2019. Fueron capturados 602 individuos, de los cuales, 26 especies son nuevos registros para el estado. Para el Amapá se registraron 58 especies de Zygoptera, insertadas en 27 géneros y siete familias. Coenagrionidae, con 45 especies registradas, comprendió la mayor abundancia durante el estudio con aproximadamente el 81% de los organismos capturados. El presente trabajo incrementa en 44% el número de especies de Zygoptera para el estado, los resultados encontrados adquieren alta relevancia en el reconocimiento de la diversidad del orden Odonata en Amapá y el bioma amazónico. The Amapá is located in northern Brazil, numerous groups are little studied in the state, among them the order Odonata. The objective of the present work is to present data on the diversity of Amapá Damselflies. Sampling was carried out between the years 2018 and 2019. Were captured 602 individuals, of which, 26 species are new records for the state. The Amapá has so far registered 58 species of Zygoptera, inserted in 27 genera and seven families. Coenagrionidae, with 45 species registered comprised the highest abundance during the study with approximately 81% of the captured organisms. The present work increases the number of species of zygopterans by 44% for the state, thus, the results found acquire high relevance in the recognition of the diversity of the order Odonata in Amapá and the Amazon biome.