No abstract

Metasurface-based lenses (metalenses) offer specific conceptual advantages compared to ordinary refractive lenses. For example, it is possible to tune the focal length of a metalens doublet by varying the relative angle between the two metalenses while fixing their distance, leading to an extremely compact zoom lens. An improved polarization-insensitive design based on silicon-nanocylinders on silica substrates is presented. This design is realized and characterized experimentally at 1550 nm wavelength. By varying the relative angle between the metalenses in steps of 10 degrees, tuning of the doublet focal length is demonstrated from −54 mm to ±3 mm to +54 mm. This results in a zoom factor of an imaging system varying between 1 and 18. For positive focal lengths, the doublet focusing efficiency has a minimum of 34% and a maximum of 83%. Experiment and theory are in very good agreement.

This paper describes work we have done in developing an insertable surgical imaging device with multiple degrees-of-freedom for minimally invasive surgery. The device is fully insertable into the abdomen using standard 12mm trocars. It consists of a modular camera and lens system which has pan and tilt capability provided by 2 small DC servo motors. It also has its own integrated lighting system that is part of the camera assembly. Once the camera is inserted into the abdomen, the insertion port is available for additional tooling, motivating the idea of single port surgery. A third zoom axis has been designed for the camera as well, allowing close-up and faraway imaging of surgical sites with a single camera unit. In animal tests with the device we have performed surgical procedures including cholecystectomy, appendectomy, running (measuring) the bowel, suturing, and nephrectomy. The tests show that the new device is:

Cell fate determination factor dachshund1 (DACH1) is a chromosome-associated protein that regulates cellular differentiation throughout development. Recent genome-wide association studies have show that missense mutation in DACH1 leads to hereditary renal hypodysplasia. Renal DACH1 expression can be used to estimate glomerular filtration rate (eGFR). We firstly characterized the function of DACH1 in normal and diseased renal tissue using immunohistochemistry to assess DACH1 in human renal biopsy specimens from 40 immunoglobulin A nephropathy (IgAN) patients, 20 idiopathic membranous nephropathy (IMN) patients, and 15 minimal change disease (MCD) patients. We found that DACH1 expression was decreased in the nephropathy group relative to healthy controls. DACH1 staining in the glomerulus correlated positively with eGFR (r = 0.41, p < 0.001) but negatively with serum creatinine (r = −0.37, p < 0.01). In vitro, DACH1 overexpression in human podocytes or HK2 cells decreased expression of cyclin D1, but increased expression of p21 and p53, which suggested that DACH1 overexpression in human podocytes or HK2 cells increased the G1/S phase or G2/M cell arrest. Together, These findings indicate that DACH1 expression is decreased in glomerulopathy imply a potential role for DACH1 in the this development of human chornic glomerulopathy. These data suggest that DACH1 is a potential a marker of disease progression and severity for glomerular diseases.