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When the sensitivity of Micrococcus luteus ATTC 9341 to streptomycin, erythromycin, oleandomycin and spiramycin was tested by an agar diffusion method using antibiotic impregnated filter paper disks on unbuffered Penassay Seed Agar two zones of inhibition were observed around the disks after an incubation period of 24 hours at 30d̀ C. The pH of the M. luteus seeded Penassay Seed Agar was measured before and after 24 hours incubation at 30d̀ C and found to be 6.6 and 8.7, respectively. When the Penassay Seed Agar was buffered to pH 6.1 and the sensitivity of the microbe to the antibiotics was tested as before no double zones of inhibition could be observed. The phenomenon of the double zones of inhibition may possibly be due to the pH increase of the medium from a relatively low level to the optimum range of activities of the antibiotics during the incubation period.

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Absiraci: 24 male NMRI mice weighing about 17 g each were divided into two equal groups. Group 1 was treated intraperitoneally with methyl mercury nitrate. The dose was 0.3 mg Hglkg in 0.5 ml water. Group 2, which was kept as a control, was treated with 0.5 ml water intraperitoneally. After 7 days all the mice were sacrificed. As seen by electron microscopy, the smooth endoplasmic reticulum was proliferated and vesicular in all the mice treated with methyl mercury. The mean liver microsomal protein content was significantly increased in group 1 as compared to group 2 (the controls).

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