BackgroundOvarian cancer (OC) is associated with high mortality in gynecological oncology; this is mainly due to the low diagnosis rate. Exosomal miRNA has demonstrated potential as a tumor biomarker. We aimed to explore the diagnostic potential of serum exosomal miR-1307 and miR-375 for OC.MethodsThe first six candidate miRNAs were selected from the previous literature. The relative quantification of qRT-PCR was used to screen for the stability of exosomal miRNAs, followed by validation of the cohort. ROC analysis was employed to analyze the specificity and sensitivity of exosomal miRNA.ResultsMiR-1307 and miR-375 were confirmed stably existing in serum exosomes of OC. Moreover, miR-1307 and miR-375 were both significantly up-regulated in serum exosomes of OC compared to ovarian benign and healthy groups. The overexpressed miRNAs showed independent diagnostic power and enhanced the diagnostic accuracy of traditional biomarkers when combined with CA-125 and HE4. MiR-1307 was associated with tumor staging, and miR-375 was associated with lymph node metastasis of OC.ConclusionOur results suggest that serum exosomal miR-1307 and miR-375 could serve as potential tumor biomarkers to improve diagnostic efficiency for OC.

The TM-doped Si-based semiconductor clusters have aroused increased attention in a lot of fields. Here, we have performed a structural search of the global minimum for neutral and anionic Si n and La2Si n (n = 1–12) clusters by utilizing the efficient CALYPSO structural searching method with subsequent DFT optimization at the B3LYP level. A large population of low energetic and stable clusters is obtained, and then, the most stable isomers for different cluster sizes are confirmed in light of their total energy and PES spectra. The effect of dopants on the geometries and electronic properties of silicon clusters and their anions are investigated. It is found that the La atoms as well as charges generate distinct influences on them. In all most stable structures of La2Si n 0/–, the impurity La atoms prefer the convex-capped positions of the skeleton and act as the electron donators. The analysis of stability reveals that La2Si3 with a bitriangular pyramid framework is very stable across the cluster size range of n = 1–12. The reason might be that there is pd hybridization between the Si and La atoms leading to strong Si–La bonds in the La2Si3 cluster. This finding is supported by the multicenter bonds and the Mayer bond order analysis.

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