Abstract:In order to get products with antioxidant activity from Arca subcrenata Lischke, the optimal hydrolase and hydrolysis conditions were investigated in the paper. Three proteases (neutrase, alcalase and papain) were applied to hydrolyze the homogenate of A. subcrenata. An orthogonal design was used to optimize hydrolysis conditions, and the pH-stat methods was used to determine the degree of hydrolysis. Viewed from the angle of reducing power, such as scavenging activities against α,α-diphenyl-β-picrylhydrazyl (DPPH) radical and hydrogen peroxide, the antioxidant activities of the alcalase hydrolysate (AH) were superior to neutrase hydrolysate (NH) and papain hydrolysate (PH), and its EC 50 values in DPPH radical and hydrogen peroxide scavenging effect were 6.23 mg/ml and 19.09 mg/ml, respectively. Moreover, compared with products hydrolyzed by neutrase and papain, the molecular mass of AH was lower and its content of amino acid of peptides was higher. Therefore, alcalase was selected as the optimal enzyme to produce active ingredients since its hydrolysate exhibited the best antioxidant activity among them and possessed large amount of potential active peptides.

One remaining problem of unitarity cut method for one-loop integral reduction is that tadpole coefficients can not be straightforward obtained through this way. In this paper, we reconsider the problem by applying differential operators over an auxiliary vector R. Using differential operators, we establish the corresponding differential equations for tadpole coefficients at the first step. Then using the tensor structure of tadpole coefficients, we transform the differential equations to the recurrence relations for undetermined tensor coefficients. These recurrence relations can be solved easily by iteration and we can obtain analytic expressions of tadpole coefficients for arbitrary one-loop integrals.

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