No abstract

BackgroundGlobal warming is characterized by not only an increase in the daily mean temperature, but also a diel asymmetric pattern. However, most of the current studies on climate change have only concerned with the mean values of the warming trend. Although many studies have been conducted concerning the responses of insects to climate change, studies that address the issue of diel asymmetric warming under field conditions are not found in the literature.Methodology/Principal FindingsWe conducted a field climate manipulative experiment and investigated developmental and demographic responses to diel asymmetric warming in three grasshopper species (an early-season species Dasyhippus barbipes, a mid-season species Oedaleus asiaticus, and a late-season species Chorthippus fallax). It was found that warming generally advanced the development of eggs and nymphs, but had no apparent impacts on the hatching rate of eggs, the emergence rate of nymphs and the survival and fecundity of adults in all the three species. Nighttime warming was more effective in advancing egg development than the daytime warming. The emergence time of adults was differentially advanced by warming in the three species; it was advanced by 5.64 days in C. fallax, 3.55 days in O. asiaticus, and 1.96 days in D. barbipes. This phenological advancement was associated with increases in the effective GDDs accumulation.Conclusions/SignificanceResults in this study indicate that the responses of the three grasshopper species to warming are influenced by several factors, including species traits, developmental stage, and the thermal sensitivity of the species. Moreover, species with diapausing eggs are less responsive to changes in temperature regimes, suggesting that development of diapausing eggs is a protective mechanism in early-season grasshopper for avoiding the risk of pre-winter hatching. Our results highlight the need to consider the complex relationships between climate change and specificity responses of invertebrates.

Grasshopper eggs overwinter in soil for almost half a year. Changes in soil temperature and moisture have a substantial effect on grasshopper eggs, especially temperature and moisture extremes. However, the combinatorial effect of temperature and moisture on the development and survival of grasshopper eggs has not been well studied. Here, we examined the effects of different soil moistures (2, 5, 8, 11, 14% water content) at 26°C and combinations of extreme soil moisture and soil temperature on the egg development and survival of three dominant species of grasshopper (Dasyhippus barbipes, Oedaleus asiaticus, and Chorthippus fallax) in Inner Mongolian grasslands. Our data indicated that the egg water content of the three grasshopper species was positively correlated with soil moisture but negatively correlated with hatching time. The relationship between hatching rate and soil moisture was unimodal. Averaged across 2 and 11% soil moisture, a soil temperature of 35oCsignificantly advanced the egg hatching time of D. barbipes, O. asiaticus, and C. fallax by 5.63, 4.75, and 2.63 days and reduced the egg hatching rate of D. barbipes by 18%. Averaged across 26 and 35°C, 2% soil moisture significantly delayed the egg hatching time of D. barbipes, O. asiaticus, and C. fallax by 0.69, 11.01, and 0.31 days, respectively, and decreased the egg hatching rate of D. barbipes by 10%. The hatching time was prolonged as drought exposure duration increased, and the egg hatching rate was negatively correlated with drought exposure duration, except for O. asiaticus. Overall, the combination of high soil temperature and low soil moisture had a significantly negative effect on egg development, survival, and egg hatching. Generally, the response of grasshopper eggs to soil temperature and moisture provides important information on the population dynamics of grasshoppers and their ability to respond to future climate change.