BackgroundInvestigating the factors that influence Acinetobacter baumannii(Ab) adhesion/invasion of host cells is important to understand its pathogenicity. Metal cations have been shown to play an important role in regulating the biofilm formation and increasing the virulence of Ab; however, the effect of calcium on host-bacterial interaction has yet to be clarified. Here, the dynamic process of the interaction between Ab and human respiratory epithelial cells and the effect of calcium on host-bacterial interaction were explored using microscopic imaging, quantitative PCR and real time cellular analysis (RTCA).ResultsThe concentration of calcium, multiplicity of infection and co-culture time were all demonstrated to have effects on host-bacterial interaction. A unique “double peak” phenomenon changed to a sharp “single peak” phenomenon during the process of Ab infection under the effect of calcium was observed in the time-dependent cell response profiles. Moreover, calcium can increase Ab adhesion/invasion of epithelial cells by regulating the expression of Ab-related genes (ompA, bfmRS, abaI).ConclusionsEffective control of calcium concentrations can provide new approaches for the prevention and treatment of multi-drug resistant Ab.

Background: Investigating the factors that influence Acinetobacter baumannii (Ab) adhesion/invasion of host cells is important to understand its pathogenicity. Metal cations have been shown to play an important role in regulating the biofilm formation and increasing the virulence of Ab; however, the effect of calcium on host-bacterial interaction has yet to be clarified. Here, the dynamic process of the interaction between Ab and human respiratory epithelial cells and the effect of calcium on host-bacterial interaction were explored using the technologies of microscopic imaging, quantitative PCR and real time cellular analysis (RTCA). Results: The concentration of calcium, multiplicity of infection and co-culture time were demonstrated to have effects on host-bacterial interaction. A unique "double peak" phenomenon changed to a sharp "single peak" phenomenon during the process of Ab infection under the effect of calcium were determined based on the time-dependent cell response profiles. Moreover, calcium can increase Ab adhesion/invasion of epithelial cells by regulating the expression of Ab-related genes ( ompA , bfmRS , abaI ). Conclusions: Effective control of calcium concentrations can provide new ideas for the prevention and treatment of multi-drug resistant Ab.

Background: Investigating the factors that influence Acinetobacter baumannii(Ab) adhesion/invasion of host cells is important to understand its pathogenicity. Metal cations have been shown to play an important role in regulating the biofilm formation and increasing the virulence of Ab; however, the effect of calcium on host-bacterial interaction has yet to be clarified. Here, the dynamic process of the interaction between Ab and human respiratory epithelial cells and the effect of calcium on host-bacterial interaction were explored using microscopic imaging, quantitative PCR and real time cellular analysis (RTCA). Results: The concentration of calcium, multiplicity of infection and co-culture time were all demonstrated to have effects on host-bacterial interaction. A unique "double peak" phenomenon changed to a sharp "single peak" phenomenon during the process of Ab infection under the effect of calcium was observed in the time-dependent cell response profiles. Moreover, calcium can increase Ab adhesion/invasion of epithelial cells by regulating the expression of Ab-related genes (ompA, bfmRS, abaI). Conclusions: Effective control of calcium concentrations can provide new approaches for the prevention and treatment of multi-drug resistant Ab.

Background Investigating the factors that influence Acinetobacter baumannii (Ab) adhesion/invasion into host cells is important to understand its pathogenicity. Metal cations have been shown to play an important role in regulating the biofilm formation and increasing the virulence of Ab; however, the effects of calcium on host-bacterial interactions have yet to be clarified. Here, the dynamic process of the interactions between Ab and human respiratory epithelial cells and the effects of calcium on host-bacterial interactions were explored using the technologies of microscopic imaging, quantitative PCR and real time cellular analysis (RTCA).Results The concentration of calcium, multiplicity of infection and co-culture time were demonstrated to have effects on host-bacterial interactions. A unique "double peak" phenomenon changed to a sharp "single peak" phenomenon during the process of Ab infection under the effects of calcium were determined based on the time-dependent cell response profiles. Moreover, calcium can increase Ab adhesion/invasion of epithelial cells by regulating the expression of Ab-related genes ( ompA , bfmRS , abaI ).Conclusions Effective control of calcium concentrations can provide new ideas for the prevention and treatment of multi-drug resistant Ab.