The aim of this study was to assess the prevalence and associated factors of alcohol use among women attending Antenatal Care in Gedeo zone rural health centers (Southern Ethiopia). This was a cross-sectional study conducted among randomly selected 718 pregnant women attending Antenatal Care. Alcohol Use Disorder Identification Test-C was used to assess alcohol consumption. Variables with p-values of < 0.05 in the multivariable logistic regression were considered as having a statistically significant association with alcohol use. Results: The prevalence of alcohol use among pregnant women attending antenatal care service was 8.1% with 95% CI (6.3-10.0). Unplanned pregnancy [AOR = 2.12, 95% CI (1.20, 3.73)], abortion history [AOR = 2.40, 95% CI (1.16, 4.96)], pre pregnancy alcohol use [AOR 2.17, 95% CI (1.18, 4.00)] and mental distress [AOR = 3.50, 95% CI (1.99, 6.15)] were variables found to have a statistically significant association with alcohol use. This calls a holistic and multi modal approach for the prevention, early identification and intervention of alcohol use during pregnancy. More emphasis should also be given for pregnant women with unplanned pregnancy, history of abortion, pre pregnancy alcohol use and mental distress.