No abstract

No abstract

Laccases produced by Trametes sp. are extracellular multi-copper oxidases, capable of oxidising large number of xenobiotics including dyes. Challenges often encountered in large-scale utilisation include the high cost of production, low yield and unstable enzymes. Lignocellulosics which abound locally are suitable low-cost alternative substrates for laccase production in Solid State Fermentation. Trametes sp. isolate B7 (GenBank accession number MK024175) was isolated and identified using molecular techniques. The study optimised laccase production using saw-dust of Terminalia superba as well as the application of same for dye decolourisation. Optimal pH and temperature were 5.0 (2356 U/mL) and 25°C (2395 U/mL) respectively. Cu 2+ , Ca 2+ , Mn 2+ and ammonium chloride induced high laccase production at 1-2 mM (2379 U/mL), 3-4 mM (2385 U/mL), 60 mM (2026 U/mL) and 0.3 g/L (2024 U/mL) respectively. Optimum incubation time, carbon Original Research Article

No abstract

No abstract