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Background: Thailand government has enacted a ban on vaping for eight years, but the number of vapers has continued to increase, particularly among teenagers. This study aimed to evaluate undergraduate pharmacy students' knowledge and attitudes toward e-cigarette use in Thailand. Method: This descriptive cross-sectional study was performed between January and March 2022. The participants were selected from 14 universities of Thailand. For data collection, a questionnaire was developed online using Google Form. The participants had to complete the questionnaire within 5 to 10 days the via s. Chi-square and binary logistic analysis were used to assess the association between variables. Results: A total of 507 participants were recruited, The participants' mean age was 20.6 ± 1.9 years. About 98.6% of the participants knew about e-cigarette, and 74.4% had never been taught about e-cigarettes. Moreover, 68.8% of the participants declared that people around them were smokers. Logistic regression analysis indicated that the participants who declared that they were not sure if e-cigarettes contains nicotine or not were 10.5 more likely to consume e-cigarettes (AOR = 10.5; 95 % CI 3.130-35.181; P ˂0.001). Male participants who were at the academic year of three and four were more likely to use e-cigarettes than female students who were at the academic year of five and six (