The last decade has seen an exponential increase in the application of unmanned aerial vehicles (UAVs) to ecological monitoring research, though with little standardisation or comparability in methodological approaches and research aims. We reviewed the international peer-reviewed literature in order to explore the potential limitations on the feasibility of UAV-use in the monitoring of ecological restoration, and examined how they might be mitigated to maximise the quality, reliability and comparability of UAV-generated data. We found little evidence of translational research applying UAV-based approaches to ecological restoration, with less than 7% of 2133 published UAV monitoring studies centred around ecological restoration. Of the 48 studies, > 65% had been published in the three years preceding this study. Where studies utilised UAVs for rehabilitation or restoration applications, there was a strong propensity for single-sensor monitoring using commercially available RPAs fitted with the modest-resolution RGB sensors available. There was a strong positive correlation between the use of complex and expensive sensors (e.g., LiDAR, thermal cameras, hyperspectral sensors) and the complexity of chosen image classification techniques (e.g., machine learning), suggesting that cost remains a primary constraint to the wide application of multiple or complex sensors in UAV-based research. We propose that if UAV-acquired data are to represent the future of ecological monitoring, research requires a) consistency in the proven application of different platforms and sensors to the monitoring of target landforms, organisms and ecosystems, underpinned by clearly articulated monitoring goals and outcomes; b) optimization of data analysis techniques and the manner in which data are reported, undertaken in cross-disciplinary partnership with fields such as bioinformatics and machine learning; and c) the development of sound, reasonable and multi-laterally homogenous regulatory and policy framework supporting the application of UAVs to the large-scale and potentially trans-disciplinary ecological applications of the future.

Monitoring is a crucial component of ecological recovery projects, yet it can be challenging to achieve at scale and during the formative stages of plant establishment. The monitoring of seeds and seedlings, which represent extremely vulnerable stages in the plant life cycle, is particularly challenging due to their diminutive size and lack of distinctive morphological characteristics. Counting and classifying seedlings to species level can be time-consuming and extremely difficult, and there is a need for technological approaches offering restoration practitioners with fine-resolution, rapid and scalable plant-based monitoring solutions. Unmanned aerial vehicles (UAVs) offer a novel approach to seed and seedling monitoring, as the combination of high-resolution sensors and low flight altitudes allow for the detection and monitoring of small objects, even in challenging terrain and in remote areas. This study utilized low-altitude UAV imagery and an automated object-based image analysis software to detect and count target seeds and seedlings from a matrix of non-target grasses across a variety of substrates reflective of local restoration substrates. Automated classification of target seeds and target seedlings was achieved at accuracies exceeding 90% and 80%, respectively, although the classification accuracy decreased with increasing flight altitude (i.e., decreasing image resolution) and increasing background surface complexity (increasing percentage cover of non-target grasses and substrate surface texture). Results represent the first empirical evidence that small objects such as seeds and seedlings can be classified from complex ecological backgrounds using automated processes from UAV-imagery with high levels of accuracy. We suggest that this novel application of UAV use in ecological monitoring offers restoration practitioners an excellent tool for rapid, reliable and non-destructive early restoration trajectory assessment.

The increasing spatial and temporal scales of ecological recovery projects demand more rapid and accurate methods of predicting restoration trajectory. Unmanned aerial vehicles (UAVs) offer greatly improved rapidity and efficiency compared to traditional biodiversity monitoring surveys and are increasingly employed in the monitoring of ecological restoration. However, the applicability of UAV-based remote sensing in the identification of small features of interest from captured imagery (e.g., small individual plants, <100 cm2) remains untested and the potential of UAVs to track the performance of individual plants or the development of seedlings remains unexplored. This study utilised low-altitude UAV imagery from multi-sensor flights (Red-Green-Blue and multispectral sensors) and an automated object-based image analysis software to detect target seedlings from among a matrix of non-target grasses in order to track the performance of individual target seedlings and the seedling community over a 14-week period. Object-based Image Analysis (OBIA) classification effectively and accurately discriminated among target and non-target seedling objects and these groups exhibited distinct spectral signatures (six different visible-spectrum and multispectral indices) that responded differently over a 24-day drying period. OBIA classification from captured imagery also allowed for the accurate tracking of individual target seedling objects through time, clearly illustrating the capacity of UAV-based monitoring to undertake plant performance monitoring of individual plants at very fine spatial scales.