Several microorganisms capable of utilizing 1-aminocyclopropane-1-carboxylate (ACPC) were isolated from soil. A bacterium which belongs to Pseudomonas accumulated cellular ƒ¿-aminobutyrate with consumption of ACPC and cells incubated with ACPC medium had the activity deaminating the substrate to form ƒ¿-ketobutyrate. An enzyme, ACPC deaminase, was highly purified and its molecular weight, substrate specificity and absorption spectrum were investigated. These results suggested that this enzyme was a pyridoxal 5•L-phosphate enzyme which has the molecular weight of 104000 and high specificity for ACPC, Km= 1.5 mm.

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