This study evaluated genetic variability and heritability in vegetative and floral characters among two minor legumes (Sphenotylis stenocarpa Ex. A. Rich. Harms and [Cajanus cajan (L.) Millsp.] of five accessions each. Six (6) floral and ten (10) vegetative characters were pooled for analysis on one hundred (100) plants of the two minor legumes. Appropriate statistical analyses were employed for comparative evaluation. Significant differences (P = 0.05) were observed in all the traits except rachis length. Principal Component Analysis (PCA) revealed significant contributions of traits in lengths of calyx lobe, stipule, rachis and petiole; also in numbers of flowers per peduncle and of main branches to the expressed variability. The cluster analysis segregated the accessions into two major cluster groups. The broad sense heritability of traits was high (>95 %). The study is important in selection of valuable traits for conservation, management of genetic resources and hybridization programs of the two species.
The study describes the impact of microwave radiation on the germination rate and growth of Abelmoschus esculentus (L.) Moench (okra). The two okra varieties, viz. Meenaxi-371 and Soniya-1402, were used for the experiment. Their seeds were exposed to 0-14 h (h) of microwave radiation (9.3 GHz). Non-irradiated seeds (0 h exposure) served as control. We found that microwave radiation on seeds resulted in an increase in their germination indices and also stimulated the concentration of β-1,3-glucanase enzyme. The results also indicate a remarkable increase in bioactive compounds such as flavonoids, polyphenols, carotenoids, chlorophyll, lutein, protein, and β-carotene in okra seedlings exposed to 8-10 h of radiation. A further dose escalation in the microwave radiation caused a decline in the germination and growth of the okra plant. The expression level of ACS4, ETR2, and ACS6 showed up-regulation under the influence of microwave irradiated conditions. Therefore, it is evident that a well-controlled and defined irradiation has the potential to enhance the overall growth and yield of selected okra varieties.
This study was carried out in the Department of Plant Science and Biotechnology, Federal University Oye Ekiti, Ekiti State. Investigation was carried out on the effects of Fresh Shoot Biomass of Chromolaena odorata on okra Abelmoschus esculentus. For the laboratory experiment, about 5g, 10g, 15g and 20g each of Chromolaena odorata was weighed into plastic bottles each containing 250ml of distilled water for 24 hours. Two millilitres of the leachate was used to water the seeds of okra that was placed in Petri dishes. Each treatment had five replicates that contained four seeds of okra (Abelmoschus esculentus). For the screening experiment, loamy top soil, fresh shoot, stems and leaves of Chromolaena odorata were collected within Federal University Oye Ekiti environment and crushed into smaller bits using mortar and pestle. Three seeds, each of okra that was planted in all the pots. The growth variables considered include radicle length, plumule length, number of roots, for the green house experiment the plant height, leaf area, stem girth, and pod weight were also observed. The leachate of Chromolaena odorata resulted to a reduction in radicle length, plumule length and number of roots. The radicle length of okra treated with leachates of 15g/250ml were adversely affected, while the plumule length of 5g/250ml, 15g/250ml and 20g/250ml greatly decreased compared to the control. The highest decrease in number of roots was noticed in the 15g/250ml concentration. The control plant had the highest plant height increased in a concentration dependent manner with exception of those treated with 200g, the leaf area of the control plant was observed to be lower than all the treatment plants, but it increased in a concentration dependent manner. Also, there was a concentration dependent increase in the steam girth of A. esculentus. The pod weight of the treated plants were lower than the control. The plant treated with 50g highest moisture content, the fat content was higher in the control and plant treated with 50g than the other treated plant. The protein content of the treatment plants was observed to be significantly higher when compared to the control plant. Crude fiber content of the treatment plant was significantly higher than the treatment plants. The ash content of the control was significantly lower than the treated plants, the carbohydrate content of the control plant increased significantly compared to the treatment plant with the lowest at 150g treated plant. The study concluded that the leachate and FSB affected the seed germination, growth and the food content of Abelmoschus esculentus.
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