We intended to perform optical and structural measurements on larval zebrafish eyes at 5 days post fertilization, that is, the earliest age at which zebrafish show visually guided behavior. However, excised larval crystalline lenses deteriorated quickly if immersed in a medium that gives good results with adult lenses from a variety of fish species. We suspected that the larvae have body fluids of lower osmolality and tested a medium with 240 mOsm, which is 75% of the established adult value of 320 mOsm. The optical quality of freshly excised and immersed lenses was used to judge the osmotic matches. In addition, we tested how well the shape of the eye is preserved in fixatives of different osmolalities. In both cases, 240 mOsm produced the best results. Immersed lenses performed better and the fixed eyes had a more natural shape. Our findings indicate that zebrafish body fluids have lower osmolality in larvae than in adults. This is probably due to an unfavorable body surface-to-volume ratio and incompletely developed regulatory mechanisms. Body fluid osmolality deviating from the adult value has to be taken into account in optical and histological work.

Arctic and boreal/temperate species are likely to be evolutionary adapted to different light regimes. Currently, the boreal/temperate Atlantic cod (Gadus morhua) is coexisting with the native polar cod (Boreogadus saida) in the Arctic waters around Svalbard, Norway. Here, we studied light/dark adaptative optical plasticity of their eye lenses by exposing fish to bright light during the polar night. Schlieren photography, high-definition laser scanning and ray tracing were used to determine the optical properties of excised crystalline lenses. Both species have multifocal lenses, an optical adaptation for improved color vision. In polar cod, the optical properties of the lens were independent of light exposure. In the more southern Atlantic cod, the optical properties of the lens changed within hours upon exposure to light, even after months of darkness. Such fast optical adjustment has previously only been shown in a tropical cichlid. During the polar night the Atlantic cod lens seems to be unregulated and dysfunctional since it had an unsuitable focal length and severe spherical aberration. We present a system, to our knowledge unique, for studying visual plasticity on different timescales in relation to evolutionary history and present the first study on the polar cod visual system.

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The petrographic composition and grain shape variability of beach gravels in the Pogorzelica–Dziwnów coast section (363.0 to 391.4 km of coastline), southern Baltic Sea, Poland were analyzed herein to characterize the lithodynamics and trends of seashore development. Gravels were sampled at 0.25 km intervals, in the midpart of the berm, following an early-autumn wave storm and before beach nourishment. Individual variations in petrographic groups along the shore were investigated. Gravel data were compared and related to coastal morpholithodynamics, seashore infrastructure, and geology of the study area. The contribution of crystalline rock gravels (igneous and metamorphic) was observed to increase along all coast sections, whereas the amount of less resistant components (limestones, sandstones, and shales) usually declined. This effect is explained by the greater wave crushing resistance of igneous and metamorphic components, compared with sedimentary components. Similarly, the gravel grain shape (mainly elongation or flattening) was observed to change, depending on resistance to mechanical destruction, or due to the increased chemical weathering in mainly the limestones, marbles, and sandstones. Observed increase in contribution of discoid and ellipsoid grains is a potential indicator of depositional trends along the coast sections investigated. On the other hand, increased contents of spheroidal and spindle-shaped grains may be related to erosional trends, where intensive redeposition and mechanical reworking of gravels occurs. However, due to the great number of coastal embankments, the petrographic composition and shape parameters of beach gravels do not always clearly indicate the dominant direction of longshore bedload transport. Increased amount of eroded limestone located east of Pogorzelica indicate increased erosion of glacial tills. These sediments are deposited, building the shallow foreshore, with additional redeposition of morainic material towards the shore.