Bovine intranuclear coccidiosis is caused by the protozoans Eimeria alabamensis and Cyclospora spp. Here, we characterized the disease and genetically identified the causative species in Japanese black calves with chronic and refractory watery diarrhea. Histologic examinations revealed atrophy of the jejunal villi and numerous parasites in the nucleus of epithelial cells in the jejunum. Based on molecular analyses using 18S ribosomal RNA gene-specific primers that we designed, the parasites were found to be formed in the same cluster as Eimeria subspherica in the phylogenetic tree, which was separated from those of other related Eimeria spp. These results constitute the first report of E. subspherica as a cause of bovine intranuclear coccidiosis.

No abstract

Gas-turbine combined-cycle (GTCC) power generation is clean and efficient, and its demand will increase in the future from economic and social perspectives. Raising turbine inlet temperature is an effective way to increase combined cycle efficiency and contributes to global environmental conservation by reducing CO2 emissions and preventing global warming. However, increasing turbine inlet temperature can lead to the increase of NOx emissions, depletion of the ozone layer and generation of photochemical smog. To deal with this issue, MHPS (MITSUBISHI HITACHI POWER SYSTEMS) and MHI (MITSUBISHI HEAVY INDUSTRIES) have developed Dry Low NOx (DLN) combustion techniques for high temperature gas turbines. In addition, fuel flexibility is one of the most important features for DLN combustors to meet the requirement of the gas turbine market. MHPS and MHI have demonstrated DLN combustor fuel flexibility with natural gas (NG) fuels that have a large Wobbe Index variation, a Hydrogen-NG mixture, and crude oils.