Advances in imaging systems have yielded a flood of images into the research field. A semi-automated facility can reduce the laborious task of classifying this large number of images. Here we report the development of a novel framework, CARTA (Clustering-Aided Rapid Training Agent), applicable to bioimage classification that facilitates annotation and selection of features. CARTA comprises an active learning algorithm combined with a genetic algorithm and self-organizing map. The framework provides an easy and interactive annotation method and accurate classification. The CARTA framework enables classification of subcellular localization, mitotic phases and discrimination of apoptosis in images of plant and human cells with an accuracy level greater than or equal to annotators. CARTA can be applied to classification of magnetic resonance imaging of cancer cells or multicolour time-course images after surgery. Furthermore, CARTA can support development of customized features for classification, high-throughput phenotyping and application of various classification schemes dependent on the user's purpose.

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Experts working for railway operators still have to devote much time and effort to creating plans for rolling stock allocation. In this paper, we formulate the railway rolling stock allocation problem as a set partitioning multi-commodity flow (SPMCF) problem and propose a search-based heuristic approach for SPMCF. We show that our approach can obtain an approximate solution near the optimum in shorter time than CPLEX for real-life problems. Since our approach deals with a wide variety of constraint expressions, it would be applicable to automatic development of practical plans for many railway operators.