A glycopeptide was isolated from cerumen and was assayed by determining the carbohydrate content of material which remained after proteolysis. The component consisted of hexose, peptide, galactosamine, sialic acid, sulfate and glucosamine ; hexose and peptide were main components. The separation posessed galactose and glucose as major hexose components.

A study has been made of the glycosaminoglycans and glycopeptide in the abdominal walls of the rats. The abdominal walls were separated into three layers ; dermis, adipose tissue and muscle containing fascia, and then uronic acid, sulfate, hexose and sialic acid contents were measured in each layer. Values per g of wet tissue were highest in the skin, whereas those of dry tissue were highest in the adipose tissue. Cellulose acetate strip-electrophoresis of materials in barbital buffer revealed the presence of three alcian blue positive fractions in every layer. The relative proportions of the fractions which corresponded in mobility to hyaluronic acid, to heparan sulfate and to chondroitin sulfates were approximately 40 %, 10 % and 50 %, respectively.

Five glycosaminoglycans were isolated from tryptic digest of bovine fourth gastric mucosa, and were assayed by determining the carbohydrate content of materials. Separation of these five polymers was achieved by preparative zone electrophoresis in a Pevikon C-870 block and by Dowex 1 •~ 2 column chromatography. They were identified as hyaluronic acid, heparan sulfate, chondroitin-4-sulfate, dermatan sulfate and chondroitin-6-sulfate, respectively.

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