Radioprotectors for acute injuries caused by large doses of ionizing radiation are vital to national security, public health and future development of humankind. Here, we develop a strategy to explore safe and efficient radioprotectors by combining Hantzsch’s reaction, high-throughput methods and polymer chemistry. A water-soluble polymer with low-cytotoxicity and an excellent anti-radiation capability has been achieved. In in vivo experiments, this polymer is even better than amifostine, which is the only approved radioprotector for clinical applications, in effectively protecting zebrafish embryos from fatally large doses of ionizing radiation (80 Gy X-ray). A mechanistic study also reveals that the radioprotective ability of this polymer originates from its ability to efficiently prevent DNA damage due to high doses of radiation. This is an initial attempt to explore polymer radioprotectors via a multi-component reaction. It allows exploiting functional polymers and provides the underlying insights to guide the design of radioprotective polymers.
Small-molecule
antioxidants perform poorly in vivo in combating
oxidative stress because of their low bioavailability. Water-soluble
polymeric antioxidants can overcome the limitations of small molecular
antioxidants (instability, poor water solubility, fast metabolism,
etc.), but there are only a few efficient synthesis methods to prepare
safe and effective polymeric antioxidants. In this study, we develop
a series of antioxidant polymers containing ferrocene and/or indole
moieties through the Ugi four-component reaction and simple free radical
polymerization. These polymers are screened using different criteria
to find a biocompatible antioxidative polymer that effectively inhibits
the lethal and teratogenic effects of UV-induced oxidative damage
on zebrafish embryos. This study identifies a strategy to use antioxidative
polymers in vivo, demonstrates the value of multicomponent reactions
in interdisciplinary areas, and provides the underlying insights to
guide the design of antioxidant polymeric biomaterials.
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