Bioacoustics plays an important role in the conservation of bird species. Bio-acoustic surveys based on autonomous audio recording are both cost-effective and time-efficient. However, there are many bird species with different patterns of vocalization, and it is a challenging task to deal with them. Previous studies have revealed that many authors focus on the segmentation of bird audio without considering specific patterns of bird vocalization. Based on the existing literature, currently there is no work on the segmentation of monosyllabic and multisyllabic birds, separately. Therefore, this research addresses the aforementioned concern and also proposes a collection of audio features named ‘Perceptual, Descriptive, and Harmonic Features (PDHFs)’ that gives promising results in the classification of bird vocalization. Moreover, the classification results improved when monosyllabic and multisyllabic birds were classified separately. To analyze the performance of PDHFs, different classifiers were used in which Artificial neural network (ANN) outperformed other classifiers and demonstrated an accuracy of 98%.