Gold nanoparticles due to their specific properties and function have found uses in the field of engineering to medical sciences. The Gold nanoparticles are always used in conjugation with other chemicals, metals, proteins, and other organic materials. The addition of other conjugates enhances the properties of Gold nanoparticles. As the insertion of metal nanoparticles into an organic matrix effectively increases the specific surface area of such materials, thereby enhancing the desired properties of the material. The term nanocomposite(NCs) is used for material containing an inorganic moiety, with at least one dimension in a nanometre range of 1–100 nm(nanoparticles ) and other materials like metal, ceramics, and polymers. The term “Bionanocomposite" (BNCs)has been assigned to nanocomposites containing a component of biological origin in the mixture. In this work, Bamboo (Bambusa bambos) was used as the organic matrix for the preparation of gold nanoparticle biochar (Au-NPs/BC) nanocomposite. The one-step synthesis approach was used for the treatment of Bamboo with Auric Chloride Salt at room temperature. In addition to the above process, the bamboo was also pyrolyzed at low temperature after treatment, which helped further to reduce the overall cost of the method. This made the method of preparation of the nanocomposite low cost and eco-friendly. Various analytical techniques such as Fourier transform infrared (FT-IR), X‐ray diffraction (XRD), Scanning electron microscopy (SEM), Energy dispersive X‐ray spectroscopy (EDS), and UV–Vis spectroscopy methods were used for the characterization of the synthesized nanocomposite. This nanocomposite was used for the preparation of electrodes and its electrical conductivity was tested. In this method, the nanocomposite was prepared in a good amount via a very simple methodology. The characterization revealed the presence of gold in the nanocomposite, which confirms that this method can be used for the preparation of the (Au-NPs/BC) nanocomposite.