Introduction: The purpose of this research was to evaluate the effect of life skills training of mothers of elementary school children on behavioral problems of children in Isfahan.Methods: In a cross-sectional study, individuals were enrolled by a multistage cluster sampling from 5 educational districts in Isfahan and 90 students who had behavioral problems were distinguished and included. The individuals were divided into case and control groups. The life skills were presented to the mothers of the case group during 9 sessions. Groups were compared regarding pre-test and post-test results.Results: The results showed that life skills training of mothers in the case group caused significant decrease in behavioral problems of children in comparison with the control group (P<0.05).Conclusion: This study demonstrated that life skill knowledge can help mothers to control their children’s behavioral problems and these sessions are suggested to be presented routinely at elementary schools.