FM-SRP resulted in clinical and microbiological improvement 6 weeks post-treatment, but produced a moderate systemic acute-phase response including elevated inflammatory mediators 1 day post-treatment.
The bowel sounds of 21 patients with mechanical obstruction of the intestine were studied. A computer analysis of bowel sounds was performed using a signal processor. Bowel sounds of patients were classified into 3 types (Types I, II, and III) based on the histograms, although these were not distinguishable on auscultation. The lower, peak, and upper frequencies were 173 +/- 25 Hz, 273 +/- 64 Hz, and 667 +/- 58 Hz, respectively, in Type I; 309 +/- 121 Hz, 632 +/- 94 Hz, and 878 +/- 116 Hz, respectively, in Type II; and 330 +/- 120 Hz, 612 +/- 86 Hz, and 766 +/- 82 Hz, respectively, in Type III. High frequency sounds above 900 Hz were recorded in Types I and II but not in Type III. The ratio of the operated patients was 0, 23, and 100 percent in Types I, II, and III, respectively. The intervals between the times of admission and operation were 4.3 days and 1.2 days in Types II and III, respectively. Thus, it appears that the methods described by the authors could provide a very objective assessment of the severity and help determine the treatment (conservative or operative) of each patient.
The Invader PLUS technology is a sensitive, rapid method for the detection and quantification of nucleic acid. While the original technology is based on the amplification by polymerase chain reaction (PCR) of the target sequence followed by its detection using the Invader technology, the current modification allows simultaneous PCR amplification and Invader reaction. The PCR primers and the Invader probes are designed to operate at the same temperature. This allows simpler design and faster results. This technology has been applied for the quantification of six periodontitis-related bacteria (Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Toreponema denticola, Tannerella forsythensis and Fusobacterium nucleatum). Direct comparison of this modified Invader PLUS with real-time PCR demonstrated similar linear range. Furthermore, testing of 64 volunteers showed a good correlation between both technologies with correlation factors r2 spanning between 0.827 and 0.987. We demonstrated here that the proposed improvement of the Invader PLUS allows the detection and quantification of DNA sequences using a simple design and protocol that can be implemented in clinical testing.
We investigated the prevalences and risk factors for peri-implant diseases in Japanese adult dental patients attending a follow-up visit at dental hospitals or clinics as part of their maintenance program. This cross-sectional multicenter study enrolled patients with dental implants who attended regular check-ups as part of a periodontal maintenance program during the period from October 2012 through September 2013. Patients with implants with at least 3 years of loading time were included in the study. The condition of peri-implant tissue was examined and classified into the following categories: healthy, peri-implant mucositis, and peri-implantitis. Patients were also evaluated for implant risk factors. A total of 267 patients (110 men, 157 women; mean age: 62.5 ± 10.7 years) were analyzed. The prevalence of patient-based peri-implant mucositis was 33.3% (n = 89), and the prevalence of peri-implantitis was 9.7% (n = 26). Poor oral hygiene and a history of periodontitis were strong risk factors for peri-implant disease. The present prevalences were lower than those previously reported. The quality of periodontal therapy before and after implant installation and patient compliance and motivation, as indicated by plaque control level, appear to be important in maintaining peri-implant tissue health.
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