: The purpose of the study is to investigate the expression of Osterix in the mouse orthodontic periodontal tissues using Waldo method. Immunohistochemecally in the control group, without experimental orthodontic mechanical stress, there was almost negative and/or quietly weekly positive reactions in the periodontal tissues. In the experimental group after 9 hours, orthodontic tension sties react positively. The positive reactions became stronger over time. The result suggest that Osterix expression in the orthodontic periodontal tension sides act as one of the key factors of osteagenic cell differentiatim.

: Early changes of Osterix expression were examined by immunohistochemistry (IHC) and fluorescent immunohistochemistry (FIHC) in mouse periodontal ligament exposed to experimentally induced orthodontic mechanical stress. Eight-week-old ddY mice were used as experimental animals. To provide continuous orthodontic mechanical stress on the periodontal ligament, a rubber dam sheet was placed between the upper molars of the mice. At 20 min, 1 h, 3 h, 9 h and 24 h after the insertion, related parts of the animal tissues were evaluated by IHC and FIHC regarding Osterix and Runx2 expressions. In the 3-h and 9-h experimental groups, orthodontic tension sites reacted positively. The positive reactions became weak in the experimental group at 24-h. The results suggest that Osterix acts as one of the key factors of osteogenic cell differentiation, which expresses the following after the expression of Runx2, down-steam of Runx2.

The etiology of erythematodes remains yet controversial. Various authors, was no sign of ascites. Both the spleen and liver were not palpable. No signs of tumors revealed in the abdominal region. The lymph nodes at all sites were