The investigation was carried out in focusing the genetic variability for different traits of wheat influenced by heat tolerance mechanism to find out relationships among phenological, physiological and yield contributing traits. Spring wheat cultivar of 25 genotypes were selected and cultivated under late sowing condition at the Regional Wheat Research Institute, Shympur, Rajshahi, Bangladesh from December, 2016 to April, 2017. Significant variability among the genotypes exposed for different traits related to heat tolerance. Results showed that the genotypes G24, G10, G01, G13, G16, G25 and G14 ranked as better category considering maximum number of traits in mean performance indicating their tolerance to heat stress under late sowing condition. Phenotypic variances ( 2 p σ ) of all traits were greater than those of genotypic variances ( 2 g σ ). The same trends were also found in their co-efficient of variances. The phenotypic co-efficient of variances (PCV) of all traits were greater compare to those of genotypic co-efficient of variances (GCV) and their values were closer to each other. The heading days (HD), canopy temperature at vegetative stage (CT vg ), canopy temperature at grain filling stage (CT gf ), biomass, plant height (PH), spike/m 2 (SPM), spikelet/spike (SPS), grain/ spike (GPS), thousand grain weight (TGW) and yield exhibited higher heritability ( 2 b h ) estimated under irrigated late sowing (ILS) condition. Under the same ILS condition SPAD, SPM, SPS, GPS, TGW and yield showed moderate to high genetic advance (GA) obtained through computing their mean percentage (%) and the rest traits HD, maturity days (MD), CT vg , CT gf , biomass, PH and harvest index (HI) exposed smaller genetic advance (% mean).