A new antitumor antibiotic, fredericamycin A (FCRC-A48, NSC-305263), has been isolated from a strain of Streptomyces griseus .Based on its unique ultraviolet-visible spectrum, infrared spectrum, proton and carbon-13 nuclear magnetic resonance spectra and mass spectra, it is judged to be a novel acid-base indicator type of compound.Its production, isolation and physicochemical properties are discussed. The isolation, ultraviolet-visible spectrum and some biological properties of two minor components, fredericamycin B and fredericamycin C, are also described.During the course of a screening program for new antitumor antibiotics, we isolated a strain of Streptomyces griseus (FCRC-48) from a soil sample in Frederick, Maryland. Fermentation broths of S. griseus (FCRC-48) repeatedly showed high in vitro activity against KB and P388 tumor cell lines and in vivo activity against P388 tumor cells. A complex of several biologically active compounds was isolated from acidified (pH 2.0) filtered broth by cooling to 4°C for 96 hours or by extraction with ethyl acetate. Purification of the complex resulted in the isolation of one major and two minor components.Other components were present, but were not isolated. This paper deals with the production, isolation and physicochemical characterization of fredericamycin A (FCRC-A48, NSC-305263), the major component. The ultraviolet-visible spectrum and some biological properties of the two minor components fredericamycin B and fredericamycin C are also reported. The biological properties of fredericamycin A are presented in the accompanying publication'). Materials and Methods GeneralMelting points, determined on a Koller micro hot stage apparatus, are uncorrected. Ultravioletvisible (UV-VIS) spectra were taken in methanol on a GCA/McPherson ultraviolet-visible spectrophotometer, Model EU-700-32. Infrared (IR) spectra (micro KBr pellet) were run by Mr. N. RISSER on a Perkin Elmer spectrophotometer, Model 180. Proton nuclear magnetic resonance (1H NMR) spectra were determined on a Varian HR-220 spectrometer equipped with a Nicolet Instrument Corp. TT220 Fourier transform accessory. Carbon-13 nuclear magnetic resonance (1IC NMR) spectra were run on a JEOL FX60 spectrometer. The electron impact mass spectra (EIMS) were run by Mr. S. HUANG on a Finnigan 3300 GC/MS with a 6000 MS data system at 70 eV (solid probe). Field desorption (FD) mass spectra were measured by Dr. G. MCCLUSKY on a Vg Micromass ZAB-2F mass spectrometer, equipped with a combination electron impact (El)-chemical ionization (CI)-field ionization (FI)-FD ion source
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.