This paper describes a digital beamforming (DBF) multibeam antenna that incorporates a compact digital signal processor (DSP) engine, and the results of using it in a beamforming experiment receiving a satellite signal in a land-mobile environment. A considerable reduction in the scale of the DSP engine has been achieved by using field programmable gate arrays (FPGA's). To capture a satellite signal arriving from an unknown direction, a two-dimensional (2-D) spatial FFT signal processing provides orthogonal multibeam patterns on the broad side of a planar array antenna. The experimental results demonstrated the features of coarse acquisition and tracking of a signal arrival by selecting the strongest of the beams without assistance from direction finding sensors or microwave phase shifters. The DBF multibeam antenna will provide high-quality communications and increase traffic capacity if it is applied to a high-gain mobile antennas or a multispot-beam base station antennas in cellular or satellite mobile communications.

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Despite channel proteins being important drug targets, studies on channel proteins remain limited, as the proteins are difficult to express and require correct complex formation within membranes. Although several in vitro synthesized recombinant channels have been reported, considering the vast diversity of the structures and functions of channel proteins, it remains unclear which classes of channels cell-free synthesis can be applied to. In this study, we synthesized 250 clones of human channels, including ion channel pore-forming subunits, gap junction proteins, porins, and regulatory subunits, using a wheat cell-free membrane protein production system, and evaluated their synthetic efficiency and function. Western blotting confirmed that 95% of the channels were successfully synthesized, including very large channels with molecular weights of over 200 kDa. A subset of 47 voltage-gated potassium ion channels was further analyzed using a planar lipid bilayer assay, out of which 80% displayed a voltage-dependent opening in the assay. We co-synthesized KCNB1 and KCNS3, a known heteromeric complex pair, and demonstrated that these channels interact on a liposome. These results indicate that cell-free protein synthesis provides a promising solution for channel studies to overcome the bottleneck of in vitro protein production.