Work fatigue is one factor that can cause errors at work. Burnout is an individual problem that requires physical, mental, and emotional transition due to long-term stress. Burnout is a problem for organizations that cause a decrease in work productivity. The nurse is one of the health workers who determine the quality of service in the hospital. The high workload of nurses raises errors in the actions given to patients. Objective: was to determine the relationship between burnout with nurses' work behavior in the hospital. This research was conducted on nurses working in hospitals. The sampling technique uses proportional random sampling method with a total of 44 people. Retrieval of burnout data include three domains, emotional exhaustion, depersonalization, and personal achievement. Results: The average age of nurses was 26.09 years, women (84. 1%), diploma education (97. 7%), and worked for 1-5 years (77.3%). There is a relationship between burnout and nurse's work behavior (p value <0.05). The higher the emotional exhaustion, the decreasing nurse's work behavior (r = -0,537). The higher the depersonalization, the lower the work behavior (r = -0.687), the higher the personal achievement, the lower nurse's work behavior (r = -0.439). Conclusion: There is a relationship between burnout and nurse's work behavior. The higher the emotional exhaustion, depersonalization and personal achievement, the nurse's work behavior decreases. The strongest close relationship is found in the depersonalization relationship with nurse's work behavior compared to other Burnout domains.