Objective To investigate the biocompatibility profile of a new peritoneal dialysis fluid containing glucose polymer (GPF). Design Viability and function of peripheral neutrophils (PMN) from healthy donors and cultured human peritoneal mesothelial cells were assessed in vitro after exposure to dialysis fluids. Phagocytosis, leukotriene B4 synthesis, and respiratory burst activation were measured following stimulation with serum-treated zymosan (STZ) or opsonized Staphylococcus epidermidis (S. epidermidis). Bacterial growth in the fluids was also investigated. In vivo pH equilibration of GPF and subsequent respiratory burst activation following incubation in spent dialysate were studied. Results For all the host defense parameters measured, commercial dialysis fluids (Dianeal; 1.36% and 3.86% glucose) and GPF (pH 5.2) were significantly more inhibitory than the control buffer (pH 7.3). Mesothelial cell viability was reduced by all the fluids tested irrespective of pH. Glucose polymer fluid was significantly more inhibitory than DianeaI 1.36% for STZ phagocytosis and respiratory burst activation. In contrast, it was less suppressive than DianeaI3.86% for L TB4 synthesis. For all parameters tested, except LTB4 generation, there was a marked effect of pH, with GPF being significantly more inhibitory at pH 5.2 than at pH 7.3. None of the fluids tested supported the growth of S. epidermidis, although the viable counts in GPF were significantly higher than in Dianeal. Fluid inhibition of PMN respiratory burst activation and cytotoxicity were reduced in a time-dependent manner following increasing dwell time in vivo. Conclusions GPF does not appear to be significantly different from Dianeal as far as host defense parameters are concerned. However, the cell viability and bacterial survival data suggest some possibly negative aspects of this fluid formation.
A question of importance in many parts of Britain is whether lucerne can supply a quantity of nutrients to compare favourably with that of other crops commonly grown for hay, silage or dried grass. There is also the need to discover the conditions under which lucerne can best withstand weed competition in high rainfall areas, and to determine the advantages, if any, of associating it with certain grasses or clover. The purpose of this experiment was to provide some critical data on these matters. On some of the experimental plots lucerne alone was sown, while on others various grasses and clover were sown with it. For comparative purposes grasses were also sown without lucerne, either alone or in association with clover. The experiment was designed to study the crops under three systems of management involving two, three or four cuts taken in the first harvest year. In the second harvest year a uniform system of three cuts was adopted for all the plots so as to study the residual effects of the different cutting treatments adopted in the preceding year. EXPERIMENTAL 'i'he plots under investigation were situated at Frongoch Farm of the Welsh Plant Breeding Station, on a Cardiganshire medium loam of average fertility, and were sown without a cover crop on 17 May 1949. The rates of seeding were as follows (lh. per acre):- Plot
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