This protocol describes a microfluidic platform for dynamic high-throughput analysis the phenotype of single cells. Cell-surface markers and secreted proteins are quantified and characterized by fluorescence detection using tailored immunoassays, simultaneously with measurement of other cellular characteristics, including endocytosis activity and viability.TWEET A new protocol describes a microfluidics-based assay for high-throughput interrogation of protein secretion kinetics in single cells. @BIOASTER @EyerFira @ETH_DCHAB COVER TEASER Microfluidics-based analysis of protein secretion Up to three primary research articles where the protocol has been used and/or developed.

In conversational speech, the acoustic signal provides cues that help listeners disambiguate difficult parses. For automatically parsing spoken utterances, we introduce a model that integrates transcribed text and acoustic-prosodic features using a convolutional neural network over energy and pitch trajectories coupled with an attention-based recurrent neural network that accepts text and prosodic features. We find that different types of acoustic-prosodic features are individually helpful, and together give statistically significant improvements in parse and disfluency detection F1 scores over a strong text-only baseline. For this study with known sentence boundaries, error analyses show that the main benefit of acousticprosodic features is in sentences with disfluencies, attachment decisions are most improved, and transcription errors obscure gains from prosody.

Background Septic shock patients exhibit an increased incidence of viral reactivation. Precise timing of such reactivation—as an early marker of immune suppression, or as a consequence of the later—is not known precisely. Here, using a fully designed nucleic acid extraction automated procedure together with tailored commercial PCR kits, we focused on the description of early reactivation within the first week of ICU admission of several herpes viruses and Torque Teno virus (TTV) in 98 septic shock patients. Results Most of septic shock patients had at least one viremia event during the first week (88%). TTV and herpesviruses were detected in 56% and 53% of septic shock patient, respectively. The two most frequent herpesviruses detected within the first week were EBV (35%) and HSV1 (26%). Different kinetic were observed among herpesviruses, faster for EBV and HSV1 than for CMV and HHV6. Although no association was found between herpes viremia and secondary infections, patients with herpesviridae-related viremia were more severe, e.g., higher SOFA scores and plasma lactate levels. While reactivating only 1 virus was not associated with mortality, patients with multiple viremia events had higher ICU mortality. Surprisingly, EBV + TTV early reactivation seemed associated with a lower D28 mortality. No clear association was observed between viremia and immune biomarkers. Conclusion Applying a semi-automated process of viral DNAemia determination to this cohort of 98 patients with septic shock, we observed that the number of patients with positive viremia increased during the first week in the ICU. Of note, there was no improvement in predicting the outcome when using viremia status. Nevertheless, this pilot study, introducing standardized procedures from extraction to detection, provides the basis for future standardized diagnostic criteria. A prospective longitudinal clinical study using these procedures will enable determination of whether such viremia is due to a lack of a latent virus control by the immune system or a true clinical viral infection. Electronic supplementary material The online version of this article (10.1186/s40635-019-0256-z) contains supplementary material, which is available to authorized users.

The Policy Research Working Paper Series disseminates the findings of work in progress to encourage the exchange of ideas about development issues. An objective of the series is to get the findings out quickly, even if the presentations are less than fully polished. The papers carry the names of the authors and should be cited accordingly. The findings, interpretations, and conclusions expressed in this paper are entirely those of the authors. They do not necessarily represent the views of the International Bank for Reconstruction and Development/World Bank and its affiliated organizations, or those of the Executive Directors of the World Bank or the governments they represent.

This work investigates style and topic aspects of language in online communities: looking at both utility as an identifier of the community and correlation with community reception of content. Style is characterized using a hybrid word and part-of-speech tag n-gram language model, while topic is represented using Latent Dirichlet Allocation. Experiments with several Reddit forums show that style is a better indicator of community identity than topic, even for communities organized around specific topics. Further, there is a positive correlation between the community reception to a contribution and the style similarity to that community, but not so for topic similarity.