No abstract

Cow’s milk-intolerance is a digestive problem on people who not able to digest milk. This problem may relate to the variant of (3-casein (CSN2), especially A1, suggested due to (3-casomorphins (BCM-7) formation during enzymatic digestion, for that selecting cattle free BCM-7 become a concern to produce digestive friendly milk. This study aimed to differentiate A1 and A2 allele variant of CSN2 gene in selected population of Indonesian Holstein cattle. In total 70 cows DNA were collected, and fragment of CSN2 exon 7 which contain Single Nucleotide Polymorphism (SNP) rs43703011 and rs43703013 were amplified. Variant analysis was done by mutation site analysis using PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) with Mspl restriction enzyme and DNA sequence for confirmation. Result shows 2 allele variants of (3-casein that are B type, representing A1 family variant, and A2 in mutation site rs43703013C>G. We found A2 allele in the studied population is superior in frequency than A1 (0.916 vs. 0.084). Of that, 8.6% cattle were heterozygotes that is BA2 and 91.4% were homozygotes A2A2. Cattle which carry A1 allele variant should be excluded from dairy cattle breeding program for further milk production free of BCM-7.

Toxicity testing of natural ingredients used in raw materials for making herbal medicines needs to be done to ensure its safety. In general, toxicity tests are divided into 2 types namely general toxicity and specific toxicity. One specific toxicity test is the teratogenic test. Teratogenic testing is part of a special toxicity test that is intended to determine the toxic effect of a compound on the fetus or fetus. The writing of this journal aims to explain the teratogenic toxicity testing technique by referring to the Organization for Economic Co-operation and Development (OECD) 414 and OECD 236 on herbal medicines. Some examples of herbs used for testing teratogenic toxicity as studied by Lu E. The et al. On Bougainvillea glabra (Paper Flower) with the results of a study of water extracts in purple and dark pink, B. glabra bract is not toxic to embryos, except the pink type with LC 50 85.51 ?g / mL. then there is Mindi leaf (Melia azedarach L) which was investigated by Adisti erlina sutomo et al with the results of extracting at a dose of 3.22 mg / kg bw already causing teratogenic toxicity. And there are still some herbs that are used for teratogenic toxicity testing with mixed results.

The turbidity of the Citarum River was high, fluctuating, and used as a drinking water source by PDAM (Regional Drinking Water Company). This study aimed to determine the ability of a combined up-flow roughing filter (URF) and an up-flow slow sand filter (USSF) in reducing turbidity, called multistage filtration (MSF). MSF was varied with and without settlers. Gravel diameters and perviousness: 0.5 cm was 0.243, 1 cm: 0.264, 2 cm: 0.265, 5 cm: 0.302 or 24-30% of URF’s volume. When settler was run with surface loading 0.5 m3/m2/hour, flowrate 0.67 l/minute, turbidity 321.16-3,496.53 NTU, the efficiency was 57.9-96.2%. Settler reduced turbidity significantly. URF1 enhanced turbidity removal. However, URF2, URF3, USSF were not effective. In an experiment without a settler, turbidity 130.78-533.00 NTU, but its reduction in URF1 was bad. In URF2 turbidity was almost the same as in the experiment with the settler. Efficiency was 41.9-89.1%. Here URF1 and URF2’s functions were almost the same as settler and URF1. URF3 was ineffective, can be removed. USSF was needed even though only able to reach 10-25 NTU. URF and USSF contributed to the higher efficiency of turbidity removal.

Setiap tahapan dari pengelolaan sampah mulai dari penyimpanan, pengumpulan, pengangkutan, daur ulang dan pembuangan akhir berkontribusi dalam mengemisikan gas rumah kaca (GRK). Penelitian ini bertujuan untuk menghitung potensi GRK dari pengelolaan sampah di Kabupaten Bandung pada tahun 2020 dari pembuangan akhir, pengomposan dan pembakaran terbuka. Metode yang digunakan untuk perhitungan GRK adalah berdasarkan standar dari IPCC dan lembar kerjanya. Hasil perhitungan menunjukkan persentase GRK terbesar berasal dari sampah yang dibuang ke Tempat Pemrosesan Akhir (TPA) yaitu sekitar 120.508,70 ton atau sekitar 84,55% dari total emisi GRK. Kemudian potensi GRK dari pembakaran sampah secara terbuka adalah sekitar 21.655,78 ton dan dari dari sampah yang dikompos adalah sekitar 366,42 ton. Namun masih terdapat sekitar 70% dari total timbulan sampah di Kabupaten Bandung yang belum terolah sehingga pengelolaan sampah yang lebih baik sangat dibutuhkan.