Wheatgrass is highly valuable due to its medicinal properties. Despite the medicinal properties, wheatgrass could not be part of daily diet, as it is not a regular part of diet so there is requirement to prepare food product supplemented with wheatgrass. In this study, we have prepared edible formulation based on wheatgrass (EFWG). The optimu m combination of ingredients for the preparation of wheatgrass incorporated edible formulation was determined using response surface methodology (RSM). RSM is used to analyse the effect of wheatgrass flakes, refined wheat flour, and frying time on sensory and objective (total phenolic and fiber levels) attributes of formulated food product. A central composite rotatable design was used to develop models for the sensory and objective responses. Responses were mostly affected by the changes in wheatgrass flakes level and frying time and to a lesser extent by the refined wheat flour. Responses individual graph plots of different responses were superimposed and regions meeting the maximum sensory score (7.85), total phenolic content (81.85 mg/100 g) and fiber (2.43 g) were identified at 7.00 g wheatgrass flakes, 90.98 g refined wheat flour and 3:50 min. frying time. Optimized formulation was analyzed for its nutritional composition, antinutritional factors and antioxidant properties. The optimized formulation could be recommended to all the age group but especially for children, lactating mothers and geriatric population due to its high calcium, iron and fiber content.

INTRODUCTION: Pregnancy is associated with high metabolic demand and increased demand for tissue oxygen. Consequently increased production of reactive oxygen species. This leads in increased oxidative stress and decreases antioxidant status during gestational age of normal pregnant women. Aim: Aim of this study was to evaluate the level of oxidant and antioxidant in 1st and 3rd trimester of normal pregnant women. MATERIAL AND METHODS: The present study included total 150 cases attended ANC Clinic at the department of Gynae LNMC & J K Hospital was screened for the study. Level of MDA was estimated by Jean et al and SOD was Marklund and Marklund. RESULTS: Findings were, that there was signicantly increase in Malondialdehyde levels (p<0.001) and signicantly decrease in superoxide dismutase activities (P<0.001) in 1st and 3rd trimester of normal pregnant women. Conclusion: present study concludes that there was difference in oxidative status due to dynamic changes. During pregnancy oxidative stress is increased and antioxidant decreased that can be fatal to the health of the mother and the fetus. Therefore antioxidant supplements should be prescribed in early pregnancy to prevent the overwhelming of oxidative stress in pregnant females.