UCOE vectors contain non-tissue specific chromatin-opening-elements that permit rapid expression of a protein in an integration independent manner. Efficient expression can be derived from a single copy of an integrated gene site resulting in a higher percentage of cells expressing the marker gene in the selected pool in comparison to standard non-UCOE containing vectors. This, in combination with the utilization of a serum-free, suspension adapted parent cell line allows for rapid production of large quantities of protein in a short period of time. Utilizing this system more than 300 mg of a recombinant antibody has been produced in less than 1 month from transfection pools in shake flask. Selected subclones have been scaled into small bioreactors in less than 2 months, producing significant quantities of monoclonal antibody using a protocol generic for the parent cell line. The increased efficiency obtained with the UCOE vector reduces the number of transfectants which need to be screened in order to obtain high productivity subclones. Transfection of a standard host cell line, preadapted to grow in a largescale setting, allows for rapid cell line development decreasing the transition time from research into development and manufacturing. Alternatively, the traditional approach of using a parent cell line which requires serum-free and suspension adaptation after transfection further increases the need for screening a large number of subclones, because many of the subclones will not be able to grow under conditions that allow large-scale protein production. The use of a preadapted cell line can reduce the time required to develop a cell line from months to weeks.

UCOE vectors contain non-tissue specific chromatin-opening-elements that permit rapid expression of a protein in anintegration independent manner. Efficient expression can bederived from a single copy of an integrated gene site resulting ina higher percentage of cells expressing the marker gene in theselected pool in comparison to standard non-UCOE containingvectors. This, in combination with the utilization of a serum-free, suspension adapted parent cell line allows for rapidproduction of large quantities of protein in a short period oftime. Utilizing this system more than 300 mg of a recombinantantibody has been produced in less than 1 month from transfectionpools in shake flask. Selected subclones have been scaled intosmall bioreactors in less than 2 months, producing significantquantities of monoclonal antibody using a protocol generic for theparent cell line. The increased efficiency obtained with the UCOEvector reduces the number of transfectants which need to bescreened in order to obtain high productivity subclones.Transfection of a standard host cell line, preadapted to grow in alarge-scale setting, allows for rapid cell line developmentdecreasing the transition time from research into development andmanufacturing. Alternatively, the traditional approach of using aparent cell line which requires serum-free and suspensionadaptation after transfection further increases the need forscreening a large number of subclones, because many of thesubclones will not be able to grow under conditions that allowlarge-scale protein production. The use of a preadapted cell linecan reduce the time required to develop a cell line from months toweeks.

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