Foodborne pathogens are the main threat and cause of food poisoning. The majority of food infections have been related to the biofilm formation of foodborne pathogens in the food industry. Shewanella putrefaciens (KX355803, GRD 03), a Gram-negative pathogen isolated from mackerel fish, was identified and recognized as a food spoilage bacterium and a strong biofilm producer. The adhesion or attachment ability of Shewanella putrefaciens was determined on steel, plastic, glass, PVC and wood. NB (Nutrient broth), LB (Luria-Bertani broth), TSB (Tryptic soy broth) and BHI (Brain heart infusion broth) were enriched with glucose and shows optimum for bacterial adhesion. In the microtiter plate method (MTP), the strong attachment was observed at 48 and 72 hours of incubation and significant differences were obtained at p < 0.05. As the incubation period increases, the OD value (Optical density) of samples also increase. Biofilm formation is the major cause cross-contamination, and shows resistance to certain disinfectants, which leads to environmental stress tolerance. This study suggested with optimum biofilm production of isolate from fish by using glucose enriched media on different substrates, also comparing different growth media provide a detailed idea about biofilm-forming ability at different incubation time intervals.

The outline of our work delineates the isolation and evaluation of sun screening activity of melanin producers such as Pseudomonas mosselli STGRDS1, Pseudomonas putida STGRDS3, Bacillus amyloliquefaciens STGRDV11, Bacillus subtilis STGRDV5 and Bacillus cereus STGRDT12. All of the isolates were tested against the fungal melanin STGRDM1, which was used as control throughout the study. The Sun Protection Factor (SPF) of formulated creams containing 5% and 10% of melanin was determined with values ranging from 1.96 ± 0.008 to 26.33 ± 0.061; further, the transmission spectroscopy was used to calculate the percentage of protection factor that stipulates the potentiality of pigments showing sunscreen effect.

No abstract

No abstract

Antilisterial bacteriocin producing strain were isolated from milk samples and were subjected to 16S rRNA sequencing and found to be of genus Enterococcus faecium. The bacteriocin ALC102 were partially purified by Amberlite XAD-16 adsorption followed by column chromatography. The biofilm formation capacity of Listeria monocytogenes MTCC 657 were evaluated by tube method and CV binding assay. Biofilm formation on different abiotic substrates were also evaluated. Among three substrates stainless steel had a strong biofilm formation followed by glass and aluminum foil. From the results of biofilm eradication studies, the bacteriocin ALC102 showed almost similar activity of commercial bacteriocin nisin on all the substrates at 45°C, 30°C, 4°C and -20°C. Based on CBD® biofilm eradication assay, the eradication potential of ALC102 and nisin were found to be similar on high (45°C) and freezing (-20°C) temperatures. From the study, antilisterial bacteriocin ALC102 found to be able to inhibit the biofilm formed Listeria monocytogenes MTCC 657 at different temperatures and different incubation periods (24h, 48h and 72h). The biofilm eradication potential of antilisterial bacteriocin ALC102 was similar to nisin. Neither incubation temperature nor incubation period doesn’t altered the activity of the bacteriocin. So this bacteriocin can be considered as a potential competitor in food industry and we strongly recommend the use of this bacteriocin from Enterococcus faecium GRD AA in the food preservation industry to a higher temperature (45°C) to freezing temperature (-20°C).