No abstract

The temperature dependent duplex formation of α-2′-deoxyoctathymidilate phosphorothioate(α-dTps7Tpo) with polyriboadenylate{poly(rA)} was studied and compared with the duplex formation of the corresponding α-dTpo7Tpo, β-dTpo7Tpo and β-dTps7Tpo with poly(rA). The DNA analog α-dTps7Tpo forms stable duplex with poly(rA) and the duplex exhibited greater stability compared to β-dTps7Tpo and poly(rA) duplex. The obtained results also indicate that the configuration of glycoside bond has greater effect of the stability of duplexes than the modification of internucleotide linkages.

α-2′-Deoxyoctathymydilic acid phosphorothioate analog bearing 3′-monophosphate was conveniently synthesized via phosphoramidite method using a riboadenosine attached Teflon-based solid support. The obtained oligomer exhibited enhanced stability toward the digestion by common nucleases.

Abstract. We shall show that the Pauli operator P A ¼ ðD À AÞ 2 I 2 À s Á B has no zero resonances if AðxÞ is smooth and jA j ðxÞj þ j'A j ðxÞj a Chxi Àr with r b 2.