Template reactions of 2-hydroxy-R-benzaldehyde-S-methylisothiosemicarbazones (R= 3methoxy or 4-hydroxy) with the corresponding aldehydes in the presence of FeCl 3 and NiCl 2 yielded N 1 ,N 4 -disalicylidene chelate complexes. The compounds were characterized by means of elemental and spectroscopic methods. The structure of complex 1 was determined by x-ray single crystal diffraction. Crystal data (Mo Kα; 296 K) are as follows: monoclinic space group P2 1 /c, a = 12.9857(8) Å, b = 7.8019(4) Å, c = 19.1976(12) Å, β = 101.655(5)°, Z = 4. Cytotoxic effects of the compounds were evaluated by MTT assay in K562 leukemia, ECV304 endothelial and normal mononuclear cells and DNA fragmentation analysis using the diphenylamine reaction were performed. The DNA binding capacity of thiosemicarbazones at IC 50 and different concentrations was investigated. The DNA fragmentation percentage of compound treated cells were higher than non-treated control cells but compound 3 (84%) was higher than the others. The interaction of compounds 1-4 and DNA was investigated voltammetrically by using nucleic acids modified electrodes after the double stranded fish sperm DNA (fsDNA), or poly (dA).poly (dT) was immobilized onto the surface of pencil graphite electrodes (PGEs). Accordingly, the oxidation signals of DNA bases; guanine and adenine were measured by using differential pulse voltammetry (DPV). The changes at the signals of guanine and adenine were evaluated before and after interaction process. The results indicated compound 3 was cytotoxic in very low concentrations in K562 leukemia cells unlike other cells and that could damage the DNA double stranded form, specifically the adenine base. Therefore, it may be a selective antileukemic effect and drug potential.thiosemicarbazones. Generally, the transition metals play a very important role in an organism and their complexes can interact non-covalently with nucleic acid by intercalation, groove-binding or external electrostatic binding for cations. 3,7,[19][20][21][22] There have been numerous studies in the literature investigating drug-DNA interactions by using conventional techniques such as HPLC, GC, and mass spectrometry. Considering the numerous advantages of electrochemical analysis techniques, the disadvantages of these conventional techniques arising from their complicated form of analysis have made them less preferable. Since the electroactivity of nucleic acids was discovered in the 1960s, 29 electrochemical nucleic acid biosensors, known as electrochemical genosensors, have frequently been preferred for recognition of (bio)molecule-DNA interactions. There are several research articles that investigated (bio)molecule-DNA interaction by using electrochemical techniques in the literature. [30][31][32][33][34][35][36] PGEs bring some crucial properties such as being robust, single-use, practical and having a large surface area. Their combination with electrochemical detection techniques provides fast, practical, accurate and time-saving analysis of target analytes. Moreover, th...
The S-methyl-thiosemicarbazones of the 2-hydroxy-R-benzaldehyde (R = H, 3-OH 3-OCH(3) or 4-OCH(3)) reacted with the corresponding aldehydes in the presence of FeCl(3) and NiCl(2). New ONNO chelates of iron(III) and nickel(II) with hydroxy- or methoxy-substituted N(1),N(4)-diarylidene-S-methyl-thiosemicarbazones were characterized by means of elemental analysis, conductivity and magnetic measurements, UV-Vis, IR and (1)H-NMR spectroscopies. Cytotoxic activities of the compounds were determined using K562 chronic myeloid leukemia and ECV304 human endothelial cell lines by MTT assay. It was determined that monochloro N(1)-4-methoxysalicylidene-N(4)-4-methoxysalicylidene-S-methyl-thiosemicarbazidato-iron(III) complex showed selective anti-leukemic effects in K562 cells while has no effect in ECV304 cells in the 0.53 microg/ml (IC(50)) concentrations. Also, some methoxy-substituted nickel(II) chelates exhibit high cytotoxic activity against both of these cell lines in low concentrations. Cytotoxicity data were evaluated depending on cell lines origin and position of the substituents on aromatic rings.
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