Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment (32 °C and 35 °C for 2 days) on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage (Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C), three genotypes of Turkish kale (Balkaya, Yanmaz, Karadere 077) and five commercial F 1 ornamental kale hybrids (Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome). Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F 1 at 32 °C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 °C.