The field experiment was conducted during fall season of 2020–21 at Mahatma Gandhi Gramodaya Vishwavidyalaya Chitrakoot, Satna (M.P.) to evaluate the effect of sulphur and boron on growth parameters, yield attributes and yield of mustard (Brassica juncea L.) under rainfed condition of Chitrakoot area. The experimental soil is sandy loam in texture having medium status of available sulphur and available boron. The experiment consists of 9 treatments combinations in factorial randomized block design with three replications consisted of three levels of sulphur (0, 30 and 40 kg ha-1), three boron levels (0, 2 ppm one spray and 2 ppm two spray). Mustard variety Pusa Mahak was grown with the recommended agronomic practices. On the basis of the results emanated from present investigation, it could be concluded that application of 40 kg S ha-1 + Two spray (2ppm boron) 40 DAS applied on mustard significantly increases growth parameter i.e. plant height, no. of leaves and no. of branches; yield attributes i.e. number of pods per plant, number of seeds per pod and 1000 seed weight (gm). Results showed that among the different fertility levels, application of 40 kg S ha-1 + Two spray (2ppm boron) 40 DAS significantly enhanced productivity parameter i.e. seed yield (kg ha-1) and stalk yield over the control.