Triacsins are a family of natural products having in common an N‐hydroxytriazene moiety not found in any other known secondary metabolites. Though many studies have examined the biological activity of triacsins in lipid metabolism, their biosynthesis has remained unknown. Here we report the identification of the triacsin biosynthetic gene cluster in Streptomyces aureofaciens ATCC 31442. Bioinformatic analysis of the gene cluster led to the discovery of the tacrolimus producer Streptomyces tsukubaensis NRRL 18488 as a new triacsin producer. In addition to targeted gene disruption to identify necessary genes for triacsin production, stable isotope feeding was performed in vivo to advance the understanding of N‐hydroxytriazene biosynthesis.

Triacsins are a family of natural products containing an N-hydroxytriazene moiety not found in any other known secondary metabolites. Though many studies have examined the biological activity of triacsins in lipid metabolism, the biosynthesis of triacsins has remained unknown. Here, we report the identification of the triacsin biosynthetic gene cluster in Streptomyces aureofaciens ATCC 31442. Bioinformatic analysis of the gene cluster led to the discovery of the tacrolimus producer Streptomyces tsukubaensis NRRL 18488 as a new triacsin producer. In addition to targeted gene disruption to identify necessary genes for triacsin production, stable isotope feeding was performed in vivo to advance the understanding of N-hydroxytriazene biosynthesis.

We report a simple means to build a model atomic force microscope (AFM) using 3D printing of thermoplastic materials that are commercially available. The model has many of the key parts of an actual AFM including a z-axis stage, an AFM head with a cantilever assembly, and a laser source that reflects off of the back of the cantilever. Using a magnet attached to the tip of the cantilever and a metal sample, this model AFM enables acquisition of force–distance profiles with characteristic snap-in, pull-off, separate, and contact regions. The model AFM was designed, printed, and used by first- and second-year undergraduate students. Through completion of this project, students learned scientific instrument design and construction via 3D printing and obtained first-hand practice in the measurement of force–distance profiles and the elastic constants of cantilevers. The open design of the model can easily accommodate additional capabilities in which students are interested, e.g., topographical scanning and using cantilevers made from different materials.