On présente dans cet article les résultats de la première étude transversale européenne portant sur les dimensions de la personnalité en relation avec les délits financiers commis par les cols blancs. Ce travail est un prolongement de la recherche de Collin & Schmidt (1992) sur les délits des cols blancs aux Etats-Unis. Les données ont été récoltées auprès de 150 cadres en poste dans des sociétés commerciales allemandes et de 76 délinquants en col blanc anciennement cadres. Les répondants ont rempli des échelles papier crayon mesurant l'hédonisme (l'Echelle de Valeurs de Schwartz), le sens des responsabilités (NEO-FFI), le narcissisme (DSM-III-R), la désirabilité sociale (Crowne & Marlowe), et le self-control comportemental. L'analyse de régression logistique montra que 69% de la variance était commune aux deux groupes. Le délit financier commis par un col blanc dépend du sexe (les hommes sont surreprésentés), d'un faible self-control comportemental, d'un hédonisme élevé, d'un fort narcissisme et d'un sens des responsabilités affirmé quand la désirabilité sociale est statistiquement contrôlée. Ce résultat concernant le sens des responsabilités est toutefois en contradiction avec l'interprétation que Collins et Schmidt donnent de leurs travaux (1993). On peut supposer que les délinquants en col blanc haut placés combinent malhonnêteté et sens des responsabilités élevé.
After blind intubation and patient positioning, more than one third of DLTs required repositioning. Routine bronchoscopy is therefore recommended after intubation and after patient positioning.
A method for cellular fractionation of Chiamydomonas reinhardii, SAG 11-32/b, and isolation of intact chloroplasts from synchronized cells of the alga is described. The procedure for ceil fractionation comprises essentially four steps: (1) protoplast production with autolysine; (2) lysis of the protoplasts with digitonin; (3) aggregation of broken protoplasts; and (4) separation of organelles by differential centrifugations.Replacing the differential centrifugations (step 4) by Percoll cushion centrifugations yields intact chloroplasts. Starting with 100 milliliters of an algal culture containing 3000 micrograms chlorophyll, intact chloroplasts with 100 to 200 micrograms of chlorophyll can be isolated. Envelope integrity is about 90% (ferricyanide assay). Examination of the chloroplasts by electron microscopy and marker enzyme activities indicated some mitochondrial and cytoplasmic contamination.The biochemistry and physiology of unicellular green algae have been studied intensively because these algae can be maintained easily under laboratory conditions. Cultures can be synchronized (21) and some strains grow heterotrophically as well as autotrophically and mixotrophically. Many similarities with the metabolism of higher plants have been established. The pathway of photosynthetic CO2 reduction was primarily elucidated with unicellular green algae. Enzymes of the glyoxylate cycle and glycolate metabolism, previously found in higher plants, could also be measured in algae, proving the general occurrence of these pathways in plants. Although it was possible to relate these pathways and cycles to cellular organelles in higher plants, very few investigations are reported on the compartmentation of metabolism in unicellular green algae. The first detailed study of localization of enzymes in a unicellular alga of the Chlamydomonas type was made by Kombrink and Wober (16) who were able to demonstrate the activity of starch-metabolizing enzymes in chloroplasts of Dunaliella marina. In their investigation, they used a new method involving DEAE-dextran for cell lysis.One reason for the difficulties in cell fractionation of unicellular
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