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A field experiment was conducted to determine the population build-up of mustard aphid and their natural enemies in relation to abiotic factors at TCA, Dholi during the Rabi season of 2018-19 and 2019-20 respectively. The incidence of mustard aphid started in the 2nd and 3rd SMW of 2019 and 2020 respectively and reached maximum in 8th SMW in both the years. However, the predatory activity of coccinellids, syrphid larvae and spiders were started from 3rd SW with their peak activity at 3rd and 4th week of February in both the years. Mustard aphid population exhibited positive correlation with maximum temperature (0.347 & 0.543), minimum temperature (0.317 & 0.152) and negative correlation with relative humidity (-0.083 & -0.479) during 2019 & 2020 respectively and rainfall (-0.35) during 2020. However, natural enemies viz., coccinellids, syrphid larvae and spiders exhibited positive correlation with Tmax, Tmin, rainfall and negative correlation with RH in both the years.