Termites are a common group of insects which acts as bio indicator of soil fertility. Termites play a major role in nitrogen fixation, acetogenesis, methanogenesis, soil transportation and nutrient movement. The objective of the study is to determine the physiochemical parameters of soil in comparison with termite mound soil and chemically fertilized soil and this study also deals with the isolation and identification of microorganisms from termite soil. This study also involves the action of termites in soil fertility by pot culture technique. The physical parameters such as pH, moisture, texture, electrical conductivity and chemical parameters such as carbon, nitrogen, calcium, magnesium, phosphorus and potassium showed increased value when compared with chemically fertilized soil. Organisms such as Citrobacter fruendii, Paenibacillus sp, Enterobacter sp, Lactococcus sp were isolated successfully. Pot culture technique showed that the plant grown in termite soil shows more growth compared with chemically fertilized soil. The results suggested that termite soil has more significant factors responsible for plant growth in comparison with chemically fertilized soil.