Current Fourier transform infrared spectroscopy (FTIR) systems have very good spectral resolution, but are bulky, sensitive to vibrations, and slow. We developed a new FTIR system using a microelectromechanical system (MEMS)-based lamellar grating interferometer that is fast, compact, and achromatic (i.e., does not require a beam splitter). The MEMS device has >10  mm² active surface area, up to ±325  μm mechanical displacement, and a 343 Hz resonant operation frequency. The system uses a 5 MHz bandwidth custom infrared (IR) detector and a small emission area custom blackbody source to achieve fast interferogram acquisition and compact form factor. Effects of lamellar grating period, detector size, laser reference, apodization, and averaging of data on the spectral resolution are discussed. The measurement time ranges from 1.5 to 100 ms depending on the averaging time. In the target range of 2.5-16 μm (625-4000  cm^-1) a spectral resolution of 15-20  cm^-1 is demonstrated. The measurements are shown to be stable over a long time.

Single particle level visualization of biological nanoparticles such as viruses and exosomes is challenging due to their small size and low dielectric contrast. Fluorescence based methods are highly preferred, however they require labelling which may perturb the functionality of the particle of interest. On the other hand, wide-field interferometric microscopy can be used to detect sub-diffraction limited nanoparticles without using any labels. Here we demonstrate that utilization of defocused images enhances the visibility of nanoparticles in interferometric microscopy and thus improves the detectable size limit. With the proposed method termed as Depth Scanning Correlation (DSC) Interferometric Microscopy, we experimentally demonstrate the detection of sub-35nm dielectric particles without using any labels. Furthermore, we demonstrate direct detection of single exosomes. This label-free and high throughput nanoparticle detection technique can be used to sense and characterize biological particles over a range between a few tens to a few hundred nanometers, where conventional methods are insufficient.

The afterglow emission of some gamma-ray bursts (GRBs) shows a shallow decay (plateau) phase implying continuous injection of energy. The source of this energy is very commonly attributed to the spin-down power of a nascent millisecond magnetar. The magnetic dipole radiation torque is considered to be the mechanism causing the spin-down of the neutron star. This torque has a component working for the alignment of the angle between rotation and the magnetic axis, i.e., the inclination angle, which has been neglected in modeling GRB afterglow light curves. Here, we demonstrate the evolution of the inclination angle and magnetic dipole moment of nascent magnetars associated with GRBs. We constrain the initial inclination angle, magnetic dipole moment, and rotation period of seven magnetars by modeling the seven long-GRB afterglow light curves. We find that, in its first day, the inclination angle of a magnetar decreases rapidly. The rapid alignment of the magnetic and rotation axis may address the lack of persistent radio emission from mature magnetars. We also find that in three cases the magnetic dipole moments of magnetars decrease exponentially to a value a few times smaller than the initial value. The braking index of nascent magnetars, as a result of the alignment and magnetic dipole moment decline, is variable during the afterglow phase and always greater than three.

Fluorescence based microarray detection systems provide sensitive measurements; however, variation of probe immobilization and poor repeatability negatively affect the final readout, and thus quantification capability of these systems. Here, we demonstrate a label-free and high-throughput optical biosensor that can be utilized for calibration of fluorescence microarrays. The sensor employs a commercial flatbed scanner, and we demonstrate transformation of this low cost (∼100 USD) system into an Interferometric Reflectance Imaging Sensor through hardware and software modifications. Using this sensor, we report detection of DNA hybridization and DNA directed antibody immobilization on label-free microarrays with a noise floor of ∼30 pg/mm, and a scan speed of 5 s (50 s for 10 frames averaged) for a 2 mm × 2 mm area. This novel system may be used as a standalone label-free sensor especially in low-resource settings, as well as for quality control and calibration of microarrays in existing fluorescence-based DNA and protein detection platforms.

We developed a prototype device for dynamic gaze and accommodation measurements based on 4 Purkinje reflections (PR) suitable for use in AR and ophthalmology applications. PR1&2 and PR3&4 are used for accurate gaze and accommodation measurements, respectively. Our eye-model was developed in Zemax and matches the experiments well. Our model predicts the accommodation from 25cm to infinity (>4 diopters) with better than 0,25D accuracy. We performed repeatability tests and obtained accurate gaze and accommodation estimations using 15 subjects. We are generating a large synthetic data set using physically accurate models and machine learning algorithms.