Electronic cigarette is one type of cigarette that are popular. This is because of the nicotine content in electronic cigarette liquid that can be regulated by the user so it is considered safe. The purpose of this study was to analize electronic cigarette effect on hematology profile of male Balb’C mice (Mus musculus L). This study used 24 males mice which were divided into 4 groups. Group 1 (control) was the group without electronic cigarette exposure. The group 2,3, and 4 were exposed to electronic cigarette for 1 ml, 2 ml and 4 ml liquid respectively. This study use liquid that containing 6 mg nicotin/ml. The exposure to electronic cigarette were conducted for 4 weeks. Hematology profile observation include hemoglobin level, erytrocytes count, count and types of leukocytes.The result showed that hemoglobin level and erytrocytes count was increase, leukocytes and lymphocyte count was decrease and no effect on granulocytes and MID after electronic cigarette exposure using different liquid volume. The conclusion of this study was  electronic cigarette exposure with different liquid volume as long as four weeks affect to Balb’C male mice hematology profile.